中国比较医学杂志2018，Vol.28Issue(4)：7-14,8.DOI:10.3969/j.issn.1671-7856.2018.04.002
6种光学透明化方法在大鼠脑组织块的透明效果比较
Comparison of transparent effects of six different optical clearing methods on rat brain tissues
摘要
Abstract
Objective To compare the transparency efficiency of six different optical clearing method on the rat brain tissues. Methods Brain tissue blocks of 14 SD rats were processed with iDISCO, SeeDB, CUBIC, SCALEVIEW-A2,CLARITY-CUBIC, Passive-CLARITY clearing method, respectively. Results The gray value of PBS group was 13.031 ± 0.586,that of iDISCO,SeeDB,CUBIC,SCALEVIEW-A2,CLARITY-CUBIC,passive-CLARITY clearing were 6.447 ± 0.574,11.690 ± 0.909,2.318 ± 0.986,8.118 ± 1.026,8.591 ± 0.384,4.198 ± 0.182, respectively. Except the SeeDB group(P=0.185),the rest groups showed significant differences compared with the PBS group(P< 0.01), and there were significant differences between CUBIC and other groups(P < 0.01). After the clearing treatment, the changes of tissue area ratio in the iDISCO, SeeDB, CUBIC, SCALEVIEW-A2, CLARITY-CUBIC, Passive-CLARITY method were(-30.02 ± 2.39)%,(19.74 ± 4.09)%,(14.7 ± 3.92)%,(10.7 ± 5.55)%,(23.01 ± 4.19)%,(66.51 ± 5.68)%,respectively. Each group showed a significant difference compared with the groups iDISCO and the Passive-CLARITY,P< 0.01. Conclusions Except the SeeDB method,all the clearing methods can achieve a transparent effect, while CUBIC is better than the other groups applied for rat brain tissues. The tissue block volume is shrunken after iDISCO clearing,and expanded after Passive-CLARITY processing.关键词
组织光学透明化技术/方法比较/大鼠脑组织Key words
optical tissue clearing/comparison/histology/brain/rat分类
医药卫生引用本文复制引用
欧毅超,冯展鹏,武广森,张源,包赟,邱炳辉,刘亚伟,漆松涛..6种光学透明化方法在大鼠脑组织块的透明效果比较[J].中国比较医学杂志,2018,28(4):7-14,8.基金项目
广东省省级科技计划项目(2016A020213006) （2016A020213006）
国家自然科学基金(81372692) （81372692）
国家科技支撑计划(2014BAI04B01). （2014BAI04B01）
