中国比较医学杂志2018，Vol.28Issue(4)：15-18,4.DOI:10.3969/j.issn.1671-7856.2018.04.003
心房钠尿肽对基因敲除小鼠移植黑色素瘤生长的抑制作用
Inhibitory effect of atrial natriuretic peptide(ANP)on the tumor growth of ANP knockout mouse and C57BL/6J mouse models of subcutaneous xenotransplanted melanoma
摘要
Abstract
Objective To study the effect of atrial natriuretic peptide(ANP)deletion on melanoma growth. Methods A subcutaneous xenotransplanted melanoma model was established in ANP knockout mice and C57BL/6J mice. The tumor volume was measured at the sixth day after establishment of the subcutaneous transplantation. Tumor cell proliferation and tumor-induced angiogenesis were detected by immunohistochemical staining. Results The volume and weight of xenotransplanted melanoma were significantly decreased in the ANP knockout mice compared with those in the C57BL/6J mice. The proliferative tumor cells and microvessel density were significantly decreased in the tumor tissues of ANP knockout mice compared with those in the C57BL/6J mice. Conclusions ANP deletion significantly suppresses xenotransplanted melanoma growth through inhibiting the tumor cell proliferation and angiogenesis.关键词
心房钠尿肽/心房钠尿肽敲除小鼠/黑色素瘤/肿瘤细胞增殖/肿瘤血管形成Key words
atrial natriuretic peptide/ANP/ANP knockout mice/melanoma/proliferation/angiogenesis分类
医药卫生引用本文复制引用
亓翠玲,曹静桦,何亚军,李梦诗,李倩明,杨扬,王丽京..心房钠尿肽对基因敲除小鼠移植黑色素瘤生长的抑制作用[J].中国比较医学杂志,2018,28(4):15-18,4.基金项目
国家自然科学基金(31500966,81773095) （31500966,81773095）
广州市科技计划项目(201607010135) （201607010135）
广东省科技计划项目(2015A020211029,2014A020212314). （2015A020211029,2014A020212314）
