中国比较医学杂志2018，Vol.28Issue(4)：27-32,6.DOI:10.3969/j.issn.1671-7856.2018.04.005
二烯丙基二硫对小鼠生精细胞的辐射防护作用
Protective effect of diallyl disulfide against the radiation damage in germ cells of male mice
摘要
Abstract
Objective To study the radiation-protective effect of diallyl disulfide(DADS)in germ cells of male mice. Methods Male mice were whole-body exposured to 4 Gy X-ray irradiation to establish a animal radiation damage model. Testicular histology, sperm motility and sperm deformity rate were observed, protein carbonyl content, malondialdehyde(MDA)and 8-hydroxy deoxyguanosine(8-OHdG)content were tested to assess the degrees of radiation damages of sperm cells and protective effect of DADS. Testicular tissue antioxidant enzymes such as superoxide dismutase (SOD),catalase(CAT),glutathione peroxidase(GSH-px)activity and glutathione(GSH)content were examined. The expression of Nrf2 signal protein was detected to explore the radiation-protective mechanism of DADS. Results Compared with the pure exposure group, the testicular tissue damages in the DADS pretreatment group were milder, sperm motility increased significantly(P< 0.05)and sperm deformity rate decreased(P< 0.05), and the protein carbonyl content, MDA and 8-OHdG levels significantly reduced(P< 0.05). The antioxidant indexes of SOD, CAT, GSH-px and GSH were markedly improved(P< 0.05),and the expression of Nrf2 was dramatically enhanced. Conclusions DADS has a protective effect on acute radiation injury in germ cells of male mice by means of anti-oxidative ability.关键词
二烯丙基二硫/X射线/小鼠/生精细胞/辐射防护剂Key words
diallyl disulfide/X-ray/mouse/spermatzoon/radioprotection分类
医药卫生引用本文复制引用
许帅,李细霞,王敏,李署平,廖伟雄..二烯丙基二硫对小鼠生精细胞的辐射防护作用[J].中国比较医学杂志,2018,28(4):27-32,6.基金项目
肇庆市科技创新指导类项目(20160403015) （20160403015）
肇庆医学高等专科学校中青年科技基金项目(Zqyq16-0010) （Zqyq16-0010）
肇庆医学高等专科学校2015年度校级科研项目(2015K66). （2015K66）
