中国现代医学杂志2018，Vol.28Issue(12)：1-11,11.DOI:10.3969/j.issn.1005-8982.2018.12.001
大黄素甲醚对白血病耐药细胞系K562/ADM耐药性的影响及机制研究
Reverse effect of Physcion on multi-drug resistance of leukemic drug-resistant cell line K562/ADM
摘要
Abstract
Objective To investigate the reverse effect of Physcion on multi-drug resistance of chronic myeloid leukemia (CML) cell line K562 / ADM. Methods CCK8 and clone formation assay were used to detect cellular proliferation. Flow cytometry and Hoechst 33258 were performed to determine apoptosis. Expression of related genes was measured by qRT-PCR and Western blot. Migration assay was identified by cell invasiveness assay. Results Physcion increased the sensitivity of K562 / ADM cells to ADM significantly. Fold change of sensitivity induced by Physcion was 2.03 fold and 5.3 fold at concentration of 2 and 5 μmol/L, respectively. miRNA-146a in K562 / ADM cells decreased significantly when compared with K562 cells, and overexpression of miRNA-146a increased the sensitivity to ADM. Physcion-induced upregulated expression of miRNA-146a facilitated inhibition of CXCL12 /CXCR4 signaling pathway. Conclusions Physcion reverses the multi-drug resistance of K562/ADM cells through modulating miRNA-146a mediated CXCL12 / CXCR4 signaling pathway.关键词
大黄素甲醚/miRNA-146a/CXCR4/白血病耐药Key words
Physcion/miRNA-146a/CXCR4/leukemia resistance分类
医药卫生引用本文复制引用
杨璇,高菲,刘文君..大黄素甲醚对白血病耐药细胞系K562/ADM耐药性的影响及机制研究[J].中国现代医学杂志,2018,28(12):1-11,11.基金项目
四川省基础研究项目(No:14JC0193-LH-35) （No:14JC0193-LH-35）
