中国现代医学杂志2018，Vol.28Issue(12)：17-23,7.DOI:10.3969/j.issn.1005-8982.2018.12.003
丹参酮ⅡA磺酸钠对血管紧张素Ⅱ诱导的大鼠心肌纤维化的影响
Sodium Tanshinone A Sulfonate attenuates angiotensin -induced cardiac fibrosis in rats
李泽 1孟哲 1李宇娜 1陶海龙 1白中乐 1李凌1
作者信息
- 1. 郑州大学第一附属医院 心内科(河南省高等学校临床医学重点学科开放实验室),河南 郑州 450052
- 折叠
摘要
Abstract
Objective To investigate the effect of Sodium Tanshinone ⅡA Sulfonate (STS) on angiotensin Ⅱ (Ang Ⅱ) -induced cardiac fibrosis in rats and potential mechanisms. Methods Rat model of cardiac fibrosis was established through constant introduction of Ang Ⅱ delivered byosmotic pump.Totally 40 male Sprague-Dawley rats were randomly divided into control group, Ang Ⅱ group, low-dose STS group, medium-dose STS group and high-dose STS group. STS were administered for three consecutive weeks. H&E and Masson's trichrome staining were performedfor morphological analysis. Expression levels of Keap1, Nrf2, Collagen Ⅰ & Ⅲ, antioxidant enzymes and phase Ⅱ enzymes were examined with qRT-PCR and Western blot. Malondialdehyde, glutathioneand superoxide dismutase activity in cardiac tissues were identified. Results STS treatment attenuated Ang Ⅱ-induced cardiac fibrosis. STS stimulatednuclear translocation of Nrf2 in a dose-dependent manner, promoting the transcription of antioxidant and phase Ⅱ enzymes. Enhanced anti-oxidative activities contributed to cellular protection against oxidative stress. Conclusion STS attenuates Ang Ⅱ-induced cardiac fibrosis by manipulating Nrf2 mediated antioxidation signaling pathway.关键词
丹参酮ⅡA磺酸钠/血管紧张素Ⅱ/心肌纤维化/Nrf2/氧化应激Key words
STS/angiotensin Ⅱ/cardiac fibrosis/Nrf2/oxidative stress分类
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李泽,孟哲,李宇娜,陶海龙,白中乐,李凌..丹参酮ⅡA磺酸钠对血管紧张素Ⅱ诱导的大鼠心肌纤维化的影响[J].中国现代医学杂志,2018,28(12):17-23,7.
