中国现代医学杂志2018，Vol.28Issue(12)：24-31,8.DOI:10.3969/j.issn.1005-8982.2018.12.004
利用光学相干断层扫描血管造影分析PM2.5对小鼠角膜上皮和角膜全层厚度的影响
Effect of PM2.5 on thickness of corneal epithelium and cornea in mice analyzed by optical coherence tomography angiography
姜楠 1朱佩文 1邵毅 1刘启 1韩云 2李娟 3杨启晨 2王亚虹 4袁晴 1马明洋 1叶蕾1
作者信息
- 1. 南昌大学第一附属医院 眼科,江西 南昌 330006
- 2. 厦门大学 眼科研究所,福建 厦门 361102
- 3. 陕西省西安市第四医院 眼科,陕西 西安 710004
- 4. 陕西省西安市环境监测站,陕西 西安 710054
- 折叠
摘要
Abstract
Objective To investigate the effect of PM2.5 on the thickness of corneal epithelium and cornea in mice by optical coherence tomography angiography (OCTA). Methods BALB/c male mice (18 - 21 g, 6 - 8 weeks of age) were divided into experimental group and control group (n = 16). Animals in experimental group received 5 mg/ml PM2.5 eye drops 4 times per day for both eyes while mice in control group experienced dPBS eye drops.Cornea was divided into 17 regions systemically. Thickness of corneal epithelium and corneal were measured using optical coherence tomography angiography at baseline, 1 day (D1) , 4 days (D4) , 7 days (D7), 10 days (D10), 14 days (D14) after intervention. Results No significant difference in thickness of corneal epithelium and corneal was founded between experimental group and control group (P > 0.05) at baseline and D1. Thickness of corneal epithelium in regions including IT5, I5, IN5, IT6, I6 and IN6 were increased significantly at D4 (P < 0.05), while no dramatic difference in thickness of the whole cornea were founded in experimental group (P > 0.05) compared with control group. Thickness of corneal epithelium in all areas was increased greatly (P < 0.05), and thickness of corneal in C2, IT5, I5, IN5, IT6, I6 and IN6 was enhanced obviously (P < 0.05) at D7 in experimental group when compared with control group. At D10 and D14, the average thickness of corneal epithelium and cornea was strengthened (P < 0.05) compared with that in control group. Conclusions PM2.5 causes increase of thickness in corneal epithelium and cornea in mice.关键词
光学相干断层扫描血管造影技术/PM2.5/角膜上皮厚度Key words
OCTA/PM2.5/corneal epithelial thickness分类
医药卫生引用本文复制引用
姜楠,朱佩文,邵毅,刘启,韩云,李娟,杨启晨,王亚虹,袁晴,马明洋,叶蕾..利用光学相干断层扫描血管造影分析PM2.5对小鼠角膜上皮和角膜全层厚度的影响[J].中国现代医学杂志,2018,28(12):24-31,8.
