中国现代医学杂志2018，Vol.28Issue(12)：36-42,7.DOI:10.3969/j.issn.1005-8982.2018.12.006
乳腺癌患者血清中miRNA-21、miRNA-210、miRNA-1246的表达及临床意义
Expression and diagnostic significance of miRNA-21, miRNA-210 and miRNA-1246 in breast cancer
摘要
Abstract
Objective To investigate the expression of miRNA in breast cancer (BC) patients and potential as diagnostic biomarkers. Methods Totally 48 BC patients as well as healthy volunteers were involved in this study. The levels of serum miRNA-21, miRNA-210 and miRNA-1246 were detected by RT-PCR. Diagnostic capability of miRNA were analyzed. Results Compared with normal subjects, levels of serum miRNA-21, miRNA-210 and miRNA-1246 were increased significantly in BC patients (P = 0.000), especially in patients on stage Ⅲ or patients with lymph node metastasis (P < 0.05). ROC curve analyses indicated that AUC value of miRNA-21, miRNA-210 and miRNA-1246 was 0.936 (95% CI: 0.866, 0.976), 0.980 (95% CI: 0.928, 0.998) and 0.737 (95% CI: 0.638, 0.822), respectively. The specificity of miRNA-21, miRNA-210 and miRNA-1246 was 0.854 (95% CI: 0.722, 0.939), 0.917 (95% CI: 0.800, 0.977) and 0.583 (95% CI: 0.432, 0.724), respectively. Further analyses showed that, in terms of diagnostic capability, combination of miRNA-210 and miRNA-1246 or combination of miRNA-21, miRNA-210, and miRNA-1246 is not superior to miRNA-210 only. Fisher discriminant analysis established the best diagnostic model for BC, whose specificity is 100.0%. Conclusion miRNA-21, miRNA-210 and miRNA-1246 are highly expressed in breast cancer patients. Serum miR-210 may be an ideal diagnostic biomarker for BC.关键词
乳腺癌/miRN-21/miRNA-210/miRNA-1246/诊断Key words
breast cancer/miRNA-21/miRNA-210/miRNA-1246/diagnosis分类
医药卫生引用本文复制引用
舒耀,吴斌,宋俊..乳腺癌患者血清中miRNA-21、miRNA-210、miRNA-1246的表达及临床意义[J].中国现代医学杂志,2018,28(12):36-42,7.基金项目
2015年泸州市人民政府-四川医科大学科技战略合作项目(No:2015sx-0023) （No:2015sx-0023）
