中国现代医学杂志2018，Vol.28Issue(12)：53-57,5.DOI:10.3969/j.issn.1005-8982.2018.12.009
西安市耐异烟肼的结核杆菌耐药性及分子特征分析
Drug resistance analysis and molecular characterization of Isoniazid resistant mycobacterium tuberculosis in Xi'an
摘要
Abstract
Objective To investigate the drug resistance of Isoniazid (INH)-resistant mycobacterium tuberculosis (MTB) in Xi'an and molecular characteristics. Methods Totally 117 INH-resistant MTB were isolated and subjected to molecular characterization of hotspot mutations within katG315 and inhA-15 by MAS-PCR (Multiplex allele-specific PCR) and direct sequencing. The isolates were genotyped by DTM-PCR (Deletion-targeted multiplex PCR). Results Among 117 isolated colonies, 73 were MDR-MTB (62.39%). Hotspot mutations analysis demonstrated that 81 (69.23%) isolates had mutation in the katG315 and 10 (8.55%) isolates had mutation in inhA-15 promotor region. DTM-PCR indicated that 104 isolates (88.9%) were Beijing genotype. Conclusion Beijing genotype accounts for the majority of INH-resistant isolates in Xi'an. Multidrug resistance of MTB in Xi'an is prevailing, and more than 70% INH-resistant isolates harbor mutation in katG315.关键词
结核分枝杆菌/异烟肼/耐药/基因分型Key words
mycobacterium tuberculosis/isoniazid/drug resistant/genotyping分类
医药卫生引用本文复制引用
周爱萍,张敏,李文侠,张增贤,沙莎,董鸿智,胡群英..西安市耐异烟肼的结核杆菌耐药性及分子特征分析[J].中国现代医学杂志,2018,28(12):53-57,5.基金项目
国家自然科学基金(No:81560668) （No:81560668）
西藏民族大学青年学人培育计划(No:15MYQP06) （No:15MYQP06）
西藏高校青年教师创新支持计划项目(No:QCZ2016-36) （No:QCZ2016-36）
西藏自治区科技厅重点项目(No:2015XZ01G17) （No:2015XZ01G17）
西藏文化传承发展协同创新中心课题(No:XT-ZB201609) （No:XT-ZB201609）
