中国烟草学报2018，Vol.24Issue(2)：30-38,9.DOI:10.16472/j.chinatobacco.2017.327
低温胁迫对烤烟幼苗光合荧光特性及叶片结构的影响
Eff ects of low temperature stress on photosynthetic fl uorescence characteristics and leaf tissue structure of fl ue-cured tobacco seedlings
摘要
Abstract
With the aim to investigate photosynthetic physiological mechanism of tobacco in response to low temperature stress, two cultivars of fl ue-cured tobacco seedlings (Honghuadajinyuan and K326) were treated with 4°C for 0h (CK), 3h, 6h, 12h, 24h and 48h to study the eff ect on photosynthetic parameters, chlorophyll fl uorescence parameters, leaf tissue structure and chloroplast ultrastructure under low temperature stress. Results showed that net photosynthetic rate, stomatal conductance, intercellular CO2concentration and transpiration decreased significantly, while water use efficiency and stomatal limitation increased significantly with prolonged stress time. The determination of chlorophyll fl uorescence parameters revealed that non-photochemical quenching coeffi cient increased signifi cantly, but PSⅡ maximal photochemical effi ciency, PS Ⅱ potential activity, PS Ⅱ eff ective quantum effi ciency, PS Ⅱ actual photochemical quantum effi ciency, photosynthetic electron transport rate and photochemical quenching coeffi cient decreased signifi cantly. Anatomical structure of K326 revealed that leaf thickness, palisade tissue thickness, spongy tissue thickness and palisade tissue/spongy thickness decreased signifi cantly. Ultrastructure showed that the phenomenon of plasmolysis generally appeared, chloroplasts were deformed, granules were arranged loosely, starch granules increased, and osmiophilic globule increased. Under low temperature stress, Honghuadajinyuan was more tolerant than K326, and the decrease of leave thickness and damage of chloroplast ultrastructure in tobacco seedlings were the main factors leading to the decrease of light energy conversion effi ciency and net photosynthetic rate.关键词
烟草/低温胁迫/光合作用参数/叶绿素荧光参数/叶片结构Key words
tobacco/low temperature stress/photosynthetic parameters/chlorophyll fl uorescence parameters/leaf structure引用本文复制引用
李琦瑶,陈爱国,王程栋,刘光亮,周培禄,郑璇,曾文龙,陈志厚,王树声..低温胁迫对烤烟幼苗光合荧光特性及叶片结构的影响[J].中国烟草学报,2018,24(2):30-38,9.基金项目
中央级公益性科研院所基本科研业务费专项"低温诱导苗期烟叶多酚类物质形成的分子机制"(1610232016019) （1610232016019）
龙岩市烟草公司项目"龙岩特色烟叶开发"(LYK201302) （LYK201302）
南平市烟草公司项目"抗寒优质烤烟新品种选育"(NYK2012-08-6) （NYK2012-08-6）
