中国烟草学报2018，Vol.24Issue(2)：39-47,9.DOI:10.16472/j.chinatobacco.2017.273
钾对烟草根际氮细菌、氮古菌群落及氮代谢的影响
Eff ect of diff erent potassium levels on nitrogen metabolism, nitrogen bacteria and nitrogen archaea bacteria communities in rhizosphere of tobacco plant
摘要
Abstract
To improve the quality of tobacco cultivation, eff ect of diff erent potassium levels on nitrogenous metabolism, nitrogen bacteria and nitrogen archaea bacteria communities in rhizosphere was syudied by means of high-throughput sequencing and spectrophotometric method. Specific results are followed. First, at the growth period with increasing amounts of potassium, the relative abundance of denitrifying bacteria and nitrate reducing bacteria decreased while the ability of reducing nitric nitrogen decreased. Second, available nitrogen was higher and increased by 20.9% than control when N:K2O=1:2.1. Third, the quality of tobacco which was revealed by chemical ingredient was higher when N:K2O=1:2.1. Fourth, at later maturing period, the nicotine was out of suitable range while sugar and potassium were below, which resulted in decreased upper leaves quality. But at the same time, total nitrogen decreased at N:K2O=1:3.5-4.2, along with urease activity. These results showed that diff erent potassium levels aff ected not only community structure of nitrogen bacteria and nitrogen archaea, but also nitrogen availability and enzyme activity, which further resulted in changes of leaf quality and quantity. It was suggested that N:K2O=1:2.1 (236.25kg K2O per hectare) could be applied to increase nitrogen utilization in soil, and dressing potassium fertilizer after topping would be required to altere rhizosphere condition and improve leaf quality.关键词
烟草/氮细菌/氮代谢/钾/根际Key words
tobacco/nitrogen bacteria/nitrogenous metabolism/potassium/rhizosphere引用本文复制引用
翁泽斌,彭世文,王淑民,何冬冬,郑国建,徐辰生,蔡海洋,刘泓,陈志厚..钾对烟草根际氮细菌、氮古菌群落及氮代谢的影响[J].中国烟草学报,2018,24(2):39-47,9.基金项目
烟草钾的吸收积累与调控研究,南平市烤烟减肥增效土壤培肥研究(201635070020114,NYK2016-03-03) （201635070020114,NYK2016-03-03）
