中国烟草学报2018，Vol.24Issue(2)：48-54,7.DOI:10.16472/j.chinatobacco.2017.302
低钾胁迫对烟草幼苗活性氧及抗氧化酶系统的影响
Eff ects of potassium defi ciency stress on active oxygen and antioxidant enzyme system in tobacco seedlings
摘要
Abstract
Response mechanism of crop to nutrient stress has become a new research hotspot. Effect of active oxygen (ROS) and antioxidant enzyme system in tobacco seedlings under potassium defi ciency stress needed to be examined. Solution culture experiments were carried out to study the eff ect of potassium defi ciency stress on biological characteristics and active oxygen scavenging system of tobacco varieties with diff erent potassium sensitivities at seedling stage. Results showed that dry weight, total length and volume of roots in tobacco seedlings decreased signifi cantly under potassium defi ciency treatment compared to normal nutrient. Malondialdehyde (MDA) content and superoxide anion radical (O2-) production rate in roots of potassium-sensitive tobacco were increased by 30.32% and 18.78% respectively, while there was no signifi cant diff erence in the increased scope of potassium-tolerant tobacco. Compared with normal nutrient, the protective enzyme activities increased in two tobacco varieties under potassium defi ciency stress except catalase (CAT) activities in shoots and roots of potassium-sensitive tobacco. The relative expression levels of protective enzyme activities such as superoxide dismutase (SOD), peroxidase (POD) and CAT of potassium-tolerant tobacco were higher than potassium-sensitive tobacco, especially in roots. It was indicated that under potassium defi ciency stress, potassium-tolerant tobacco had high protective enzyme expression to protect tobacco seedlings from harm of excess O2- and H2O2, thus increasing adaptability to potassium defi ciency.关键词
烟草幼苗/低钾胁迫/活性氧/抗氧化酶Key words
tobacco seedling/potassium defi ciency/ROS/antioxidant enzyme引用本文复制引用
况帅,冯迪,宋科,孟霖,宋文静..低钾胁迫对烟草幼苗活性氧及抗氧化酶系统的影响[J].中国烟草学报,2018,24(2):48-54,7.基金项目
中国农业科学院科技创新工程(ASTIP-TRIC03),国家自然科学基金青年基金(3601818)和中国农业科学院烟草研究所青年基金(2016A03) （ASTIP-TRIC03）
