中国烟草学报2018，Vol.24Issue(2)：129-134,6.DOI:10.16472/j.chinatobacco.2017.367
河南烟草镰刀菌的分子鉴定及致病性分析
Molecular identification and pathogenicity analysis of tobacco Fusarium spp.in Henan
摘要
Abstract
To identify Fusarium species in different tobacco producing areas of Henan province,tobacco plants with typical symptoms of fusarium root rot and fi eld soil were sampled to isolate pathogens by tissue separation method and soil separation method. Pure strains were identified by morphological characteristics and rDNA-ITS sequence analysis, and their pathogenicities were determined by non-invasive inoculation method. Morphological observation showed that on PDA medium, all of the 98 purifi ed stains produced white hyphae, macroconidium was sickle or matt in shape, colorless and multi-cell; microconidium was reniform, ovoid or oval in shape, colorless and most of them were single-cell. Sequence alignment indicated that the 98 isolated strains were Fusarium oxysporum, Fusarium solani, Fusarium proliferatum,Fusarium equiseti and Fusarium nematophilum.Pathogenicity analysis showed that 48 strains were pathogenic to tobacco seedlings,which were F.oxysporum,F.solani,F.proliferatum and F.equiseti.In Southern Henan,the pathogen of fusarium root rot were F.oxysporum,F.proliferatum and F.equiseti,with F.oxysporum the predominant pathogen.F.oxysporum was the pathogenic fungi in Centrol Henan,and F.solani and F.oxysporum were the pathogenic stains in Eastern and Western Henan.Therefore,F.oxysporum was the key prevention and control objects in all tobacco planting area of Hennan province,and control of F.proliferatum and F.proliferatum need to be strengthened in Southern Henan,and strict precautions measure be taken against the occurrence of F.solani.in Eastern and Western Henan.关键词
河南省/烟草/镰刀菌/分子鉴定/致病性Key words
Henan Province/Tobacco/Fusarium spp./Molecular identification/Pathogenicity引用本文复制引用
邱睿,白静科,李成军,李淑君,李小杰,陈玉国,胡亚静,刘东升..河南烟草镰刀菌的分子鉴定及致病性分析[J].中国烟草学报,2018,24(2):129-134,6.基金项目
河南省农业科学院自主创新专项基金项目"烟草真菌类病害的采集、分离及快速检测体系的建立"(2017ZC21) （2017ZC21）
河南省2017年财政预算项目"烟草真菌类病害快速检测体系的建立"(豫财预[2017]76-6) （豫财预[2017]76-6）
河南省烟草公司科学研究与技术开发重点项目"河南烟草病虫害绿色防控关键技术研究与应用"(201741000027063) （201741000027063）
