中国烟草学报2018，Vol.24Issue(2)：135-140,6.DOI:10.16472/j.chinatobacco.2017.318
烟草镰刀菌根腐病的病原鉴定
Identification of tobacco Fusarium root rot pathogen
摘要
Abstract
In order to identify tobacco root rot pathogen,31 strains of Fusarium spp.were isolated from samples of tobacco Fusarium root rot disease which were collected from the main tobacco areas of Sanming city, Fujian province from 2015 to 2017. The pathogen was identified as Fusarium commune by morphological comparison, pathogenicity determination, species-specific molecular detection and phylogenetic analysis.Micro-morphological characteristics of F.commune were similar to F.oxysporum.All 31 Fusarium spp.isolates were identified as F.commune by using specific primers;strain F-04,F-18,F-19,F-28 was clustered with F.commune and clearly distinguished from F.oxysporum by phylogenetic analysis of the rRNA intergenic spacer region gene(IGS)using Bayesian analysis.Pathogenicity of the isolates was tested by greenhouse pots and field inoculation experiments.It is the first report that F.commune caused tobacco root rot disease in China.关键词
烟草根腐病/病原菌/鉴定/共享镰刀菌Key words
tobacco root rot disease/pathogen/identification/Fusarium commune引用本文复制引用
吴安忠,程崖芝,巫升鑫,刘国坤,张绍升,肖顺..烟草镰刀菌根腐病的病原鉴定[J].中国烟草学报,2018,24(2):135-140,6.基金项目
中国烟草总公司福建省公司科技项目"烟草镰孢菌根腐病和枯萎病抗性鉴定技术体系构建与应用"(2014[180]号) （2014[180]号）
