中国药理学通报2018，Vol.34Issue(5)：651-656,6.DOI:10.3969/j.issn.1001-1978.2018.05.013
海带多糖对脂多糖致慢性炎症大鼠血管内皮的保护作用
Protective effects of laminarin on vascular endothelium in rats with chronic inflammation induced by LPS
摘要
Abstract
Aim To observe the effect of laminarin L01 on the expression of eNOS and iNOS in aorta of rats with chronic inflammation induced by LPS. Methods Chronic inflammatory rat models were prepared by tail vein injection low dose LPS(0.4 mg·kg-1) once a week for four weeks. The rats were randomly divided into five groups. After the first injection of LPS, the DXM group was intraperitoneally injected with dexam-ethasone (10 mg·kg-1). L01 high,medium and low dose groups were intraperitoneally injected with L01 (50,30,10 mg·kg-1). The LPS group was injected intraperitoneally with equal volume of normal saline once a day. Another control group, only injection of normal saline, a total of four weeks. After the last administration,the number of whole white blood cells (WBC) was counted. ELISA was used to measure the hs-CRP in serum. The expressions of eNOS,iNOS and COX-2 mRNA were detected by RT-PCR. Results After four weeks of administration of L01, the number of WBC and the level of serum hs-CRP in chronic in-flammatory rats were significantly decreased. The ex-pression of eNOS was up-regulated, and iNOS and COX-2 expressions were down-regulated. Conclusions Laminarin L01 may regulate the expression and re-lease of endothelium-derived relaxing factor stimulated by LPS,and improve the endothelium-dependent dias-tolic function of aorta, thus protecting the damage of vascular endothelium.关键词
海带多糖/慢性炎症/脂多糖/血管内皮/eNOS/iNOSKey words
laminarin L01/chronic inflammation/li-popolysaccharide/vascular endothelium/eNOS/iNOS分类
医药卫生引用本文复制引用
王靳琎,杨晓梅,苏丽万,钱航,谢露..海带多糖对脂多糖致慢性炎症大鼠血管内皮的保护作用[J].中国药理学通报,2018,34(5):651-656,6.基金项目
广西省自然科学基金资助项目(No 2015GXNSFAA139148) （No 2015GXNSFAA139148）
广西医科大学青年科学基金资助项目(No 02604001078X) （No 02604001078X）
