中国药理学通报2018，Vol.34Issue(5)：664-669,6.DOI:10.3969/j.issn.1001-1978.2018.05.015
钾通道阻滞剂四乙基铵敏感性钾电流在肺高压大鼠肺动脉平滑肌细胞中的变化
Alteration of tetraethylammonium-sensitive potassium current in pulmonary artery smooth muscle cells of pulmonary hypertension rats
摘要
Abstract
Aim To investigate the alteration of volt-age-depending potassium channel(KV) current in pul-monary arterial smooth muscle cells(PASMCs) of pul-monary hypertension (PH) rats, and the effect of tet-raethylammonium (TEA,a blocker of KV) on potassi-um channel current in different PH models. Methods The whole-cell patch clamp techniques were applied to record the KVcurrents from PASMCs cultured with Ham's F-12 (1% FBS). Furthermore, the effects of TEA on the KVcurrents were examined in different PH models. Results The whole-cell KVcurrents were ob-viously inhibited in PASMCs of chronic hypoxia (CH)and monocrotaline (MCT)-treated rats. TEA signifi-cantly decreased the whole-cell KVcurrents in PASMCs of control and PH rats,and the inhibitory effect of TEA was dramatically reduced in PH group. Conclusions The degree of the voltage-dependent potassium chan-nels opening is significantly inhibited in PASMCs of CH and MCT-treated rats,accordingly,the TEA-sen-sitive KVcurrents obviously decrease.关键词
四乙基铵/电压依赖性钾通道/肺高压/肺动脉平滑肌细胞/野百合碱/慢性低氧Key words
TEA/voltage-depending potassium chan-nel/pulmonary hypertension/pulmonary artery smooth muscle cells/monocrotaline/chronic hypoxia分类
医药卫生引用本文复制引用
张双双,穆云萍,贺志成,颜晓晓,林默君..钾通道阻滞剂四乙基铵敏感性钾电流在肺高压大鼠肺动脉平滑肌细胞中的变化[J].中国药理学通报,2018,34(5):664-669,6.基金项目
国家自然科学基金资助项目(No 31571179,31371165) （No 31571179,31371165）
福建省自然科学基金资助项目(No 2015J01313) （No 2015J01313）
