中国药理学通报2018，Vol.34Issue(5)：675-680,6.DOI:10.3969/j.issn.1001-1978.2018.05.017
吗啡预处理对NGF诱导神经细胞TRPV1通道敏化及ERK蛋白活化的影响
Effects of morphine preconditioning on TRPV1 channel current and ERK phosphorylation sensitized by NGF in neurocytes
摘要
Abstract
Aim To investigate the effects of morphine preconditioning (MPC) on transient receptor potential vanilloid 1 (TRPV1) channel current in rat dorsal root ganglia (DRG) neurons and the phosphorylation (p) of TRPV1 and extracellular regulated protein kinases (ERK) in PC12 cells that sensitized by nerve growth factor (NGF). Methods DRG neurons isolated from T2-T8 segments of 10 days old SD rat or pheochromo-cytoma (PC12) cells were seeded into 24-well plates or 6-well plates, respectively, and randomly divided into 5 groups:control group (group C), NGF sensiti-zation group (group NGF), and morphine precondi-tioning groups (group MPC 0.3, group MPC 1.0 and group MPC 3.0). DRG neurons were identified by im-munofluorescent method with neuronal specific enolase (NSE). Cells were treated by morphine, NGF and capsaicin to simulate the effects of MPC on DRG neu-rons in T2-T8 segments during myocardial ischemia reperfusion injury (IRI). Afterwards, the inward cur-rent of DRG neurons induced by capsaicin in all groups were detected by whole cell recording; the expression and phosphorylation of TRPV 1 and ERK in PC12 cells were detected by Western blot. Results DRG neu-rons survived and grew nicely,and the staining of neu-ronal specific markers,NSE,was positive. In compar-ison with group C, the inward current of group NGF was enhanced (P <0.05), while MPC inhibited the enhancement (P <0.05). The relative expression of TRPV1,p-TRPV1 and p-ERK in group NGF was up-regulated when compared with group C (P <0.05).Moreover, the up-regulation was also suppressed by MPC (P <0.05). Conclusions MPC inhibits TR-PV1 channel current sensitized by NGF in neurocytes, and the mechanism might be associated with the down-regulation of TRPV1 p-TRPV1 and p-ERK expression.关键词
吗啡/预处理/背根神经节/神经生长因子/单细胞膜片钳/细胞外信号调节激酶Key words
morphine/preconditioning/dorsal root ganglia/nerve growth factor/single cell patch clamp/extracellular regulated protein kinase分类
医药卫生引用本文复制引用
马振晓,何淑芳,邹桂昌,黄成,熊伟,张野..吗啡预处理对NGF诱导神经细胞TRPV1通道敏化及ERK蛋白活化的影响[J].中国药理学通报,2018,34(5):675-680,6.基金项目
国家自然科学基金资助项目(No 81471145) （No 81471145）
