中国药理学通报2018，Vol.34Issue(5)：680-685,6.DOI:10.3969/j.issn.1001-1978.2018.05.018
丹酚酸B通过调控SIRT1/NF-κB/p53通路减轻缺氧/复氧诱导的大鼠肝细胞损伤
Sal B attenuates rat hepatocytes injury induced by hypoxia/reoxygenation via modulation of SIRT1/NF-κB/p53 pathway
摘要
Abstract
Aim To investigate the effect of salvianolic acid B(Sal B) on the attenuation of rat hepatocyte in-jury induced by hypoxia/reoxygenation(H/R) and its possible molecular mechanism. Methods Rat hepato-cytes BRL-3A were cultured in vitro. H/R injury mod-el was established and then BRL-3A cells were pretrea-ted with Sal B. The viability of cells was measured by CCK-8 assay;the expression of ALT and AST was de-tected by microplate assay; the levels of TNF-α and IL-1β were determined by ELISA; the apoptosis was detected by flow cytometry;the protein and mRNA lev-els of SIRT1, NF-κB p65, p53, Bax and Bcl-2 were measured by Western blot and qPCR. Results H/R intervention decreased the viability and increased the apoptosis of cells;the production of ALT, AST, TNF-α and IL-1β was elevated;the protein and mRNA lev-els of SIRT1, Bcl-2 were reduced, but the levels of NF-κB p65, p53 and Bax increased. After pretreated with Sal B, the viability of cells increased while the apoptosis decreased; the expression of ALT, AST, TNF-α and IL-1β was inhibited;moreover,the protein and mRNA levels of SIRT1,Bcl-2 were enhanced,and the levels of NF-κB p65, p53 and Bax decreased sig-nificantly. Conclusion Sal B may attenuate rat hepa-tocyte injury induced by H/R via the SIRT1/NF-κB/p53 pathway.关键词
丹酚酸B/沉默信息调节因子1/核因子κBp65/p53/缺氧/复氧/BRL-3AKey words
salvianolic acid B/SIRT1/NF-κB p65/p53/hypoxia/reoxygenation/BRL-3A分类
医药卫生引用本文复制引用
万磊,陈青松,周壮,周翔宇,郑道峰,吴忠均..丹酚酸B通过调控SIRT1/NF-κB/p53通路减轻缺氧/复氧诱导的大鼠肝细胞损伤[J].中国药理学通报,2018,34(5):680-685,6.基金项目
国家自然科学基金资助项目(No 81672959) （No 81672959）
重庆市科学技术委员会基金项目(No cstc2015shmszx120019) （No cstc2015shmszx120019）
