中国药理学通报2018，Vol.34Issue(5)：686-690,5.DOI:10.3969/j.issn.1001-1978.2018.05.019
叶下珠抗补体活性研究
Anticomplementary activity and constituents of Phyllanthus urinaria
摘要
Abstract
Aim To explore the anticomplementary ac-tivity and active constituents of Phyllanthus urinaria. Methods Being guided by bioactive screening,the anticomplementary constituents of P.urinaria were iso-lated and purified by solution partition and chromato-graphic techniques,and their structures were identified by 13C-NMR spectrum and the comparison of reported data. The anticomplementary activity and possible mechanism were assayed.Results The EtOAc fraction of the methanol extract of P.urinaria was shown to be the active fraction,and two compounds were purified from the fraction and were identified as corilagin and ellagic acid.The EtOAc and the two compounds signifi-cantly inhibited the hemolysis of the complement classi-cal pathway,with IC50values of 53.77 mg·L-1, 176.54 mg·L-1and 102.23 mg·L-1,respectively. While they just showed slight effect on inhibiting the hemolysis of the complement alternative pathway. All of them affected the formation of C3 convertase of the classical pathway. Conclusions The polyphenols are main anticomplentary constituents of P. urinaria,and its mechanism is related to inhibiting formation of C3 convertase of the classical pathway.关键词
叶下珠/活性成分/抗补体/经典途径/替代途径/C3转化酶Key words
Phyllanthus urinaria/active constituents/anticomplementary activity/classical pathway/alterna-tive pathway/C3 convertase分类
医药卫生引用本文复制引用
朱芳娟,侯灵莉,孙黔云,杨庆雄..叶下珠抗补体活性研究[J].中国药理学通报,2018,34(5):686-690,5.基金项目
国家十三五重点研发计划项目(No 2016YFC0502600) （No 2016YFC0502600）
贵州省科技创新人才团队项目(黔科合平台人才[2016]5625号) （黔科合平台人才[2016]5625号）
贵州省高层次创新型人才培养项目(黔科合人才[2016]4018号) （黔科合人才[2016]4018号）
贵阳市科技计划项目(筑科合同[2017]8-2号) （筑科合同[2017]8-2号）
