中国药理学通报2018，Vol.34Issue(5)：706-712,7.DOI:10.3969/j.issn.1001-1978.2018.05.023
载腺嘌呤甘草次酸修饰透明质酸纳米粒的制备及其对肝癌细胞增殖的影响
Preparation and proliferation effect on hepatoma cells of adenine loaded glycyrrhetinic acid modified hyaluronic acid nanoparticles
摘要
Abstract
Aim To prepare hyaluronic acid nanoparti-cles(Ade/GA-HA) using glycyrrhetinic acid modified hyaluronic acid as the carrier and adenine as a model drug, and analyze their physicochemical property and proliferation effect on Bel-7402 cells. Methods Gly-cyrrhetinic acid and hyaluronic acid were combined by chemical cross-linking method, dialysis and freeze-dr-ying,based on which Ade/GA-HA was prepared using ultrasonic method, and the particle size and Zeta po-tential were determined by Malvern laser particle analy-zer,and the morphology was observed by transmission electron microscopy, and the absorbance was deter-mined by ultraviolet-visible spectrophotometer, high performance liquid chromatograph and microplate read-er to caculate drug load, encapsulation efficiency and in vitro release. MTT assays were utilized to determine the proliferation of nanoparticles treated Bel-7402 cells. Results GA-HA nanoparticles had spherical shape with a good dispersion, at diameters of 398.1 nm, of which Zeta potential was - 34.2 mV, and presented good short term stability. The drug load and encapsulation efficiency of Ade/GA-HA nanoparticles were (22.5 ± 5.8)% and (87.27 ± 0.33) %, re-spectively. Burst release was observed in Ade/GA-HA nanoparticles within 4 h, while controlled release 4 h later. Compared with free adenine,Ade/GA-HA nano-particles had a stronger inhibitory effect on cell prolif-eration with statistically significant difference. Conclu-sion GA-HA nanoparticles has excellent physico-chemical properties and meet the design requirement.关键词
甘草次酸/透明质酸/腺嘌呤/纳米粒/理化性质/细胞毒性Key words
glycyrrhetinic acid/hyaluronic acid/ade-nine/nanoparticles/physicochemical property/cyto-toxicity分类
医药卫生引用本文复制引用
吴菲,于文静,高志芹,张龙骧,李肖成,蒋彬,邹斯亦,王晨,牟文清,连波,武敬亮..载腺嘌呤甘草次酸修饰透明质酸纳米粒的制备及其对肝癌细胞增殖的影响[J].中国药理学通报,2018,34(5):706-712,7.基金项目
国家自然科学基金资助项目(No 81274093) （No 81274093）
山东省高等学校科技计划项目(No J17KA141) （No J17KA141）
