中国药理学通报2018，Vol.34Issue(5)：735-739,5.DOI:10.3969/j.issn.1001-1978.2018.05.028
人U87-MG脑胶质瘤细胞裸鼠原位移植模型的建立
Establishment of orthotopic implantation model of human U87-MG brain glioma cell line in nude mice
摘要
Abstract
Aim To establish human U87-MG glioma model in nude mice brain and to observe the characteristics of the tumor growth. Methods Human U87-MG glioma cells were cultured in vitro. 5 μL of cell suspension containing 3.0 ×1010·L-1, 4.0×1010·L-1and 5.0×1010·L-1respectively was inocula-ted into the right caudate nucleus of 18 male nude mice brain un-der the guidance of stereotaxic apparatus, separately, whereas another 6 nude mice as the control group, were inoculated into the same volume of Hanks solution. The moving and survival state of rats with gliomas were observed. The examinations of the tumors formation, volumes, metastasis and histopathology were performed and the obtained brain samples were stained with HE and immunohistochemistry. Results All the tested rats of dif-ferent inoculation doses developed brain tumors without extracra-nial metastasis. The mean survival time of three groups was (46.50 ± 3.27) d,(38.50 ± 3.28) d and (30.67 ± 3.51) d,respectively. The tumors showed the similar morphological fea-tures and immunophenotype to human glioma. There was positive expression of GFAP and S-100 in the tumors. Conclusions The orthotopic implantation model of human U87-MG glioma, by in-oculating quantitative U87-MG cells stereotaxically into the brains of the nude mice, is successfully established with 100 yield of intracranial tumor and no extracranial growth extension. It resembles the histopathological and morphological features of human glioma,which can be used as a reliable animal model for the study of the tumorigenesis, pathogenesis, biological charac-teristics and therapy of glioma.关键词
脑胶质瘤/原位移植/裸鼠/动物模型/U87-MG细胞/HE染色/免疫组化Key words
glioma/orthotopic implantation/nude mice/ani-mal model/U87-MG cells/HE staining/immunohistochemistry分类
医药卫生引用本文复制引用
王艳华,奚苗苗,文爱东,翁琰,楚建杰,李子敏,胡娜平,李会会,郑建民,张彩勤,师长宏,杨志福..人U87-MG脑胶质瘤细胞裸鼠原位移植模型的建立[J].中国药理学通报,2018,34(5):735-739,5.基金项目
国家自然科学基金资助项目(No 81303264) （No 81303264）
