重庆医学2018，Vol.47Issue(11)：1440-1443,1448,5.DOI:10.3969/j.issn.1671-8348.2018.11.003
内质网应激对大鼠局灶性脑缺血再灌注后听皮层损伤的作用
The effect of ERS on the auditory cortex injury after focal cerebral ischemia-reperfusion injury in RATS
摘要
Abstract
Objective To study the effect and mechanism of endoplamic reticulum stress on the auditory cortex injury after focal cerebral ischemia-reperfusion injury in rats.Methods 30 healthy male adult SD rats were chosen and randomly divided into 2 groups,ischemia-reperfusion(I/R) group and sham operated control,with each group 15 rats.The rats in I/R group were operated for suture-occluded method to establish middle cerebral artery occlusionmodel(MCAO),with ischemia for 60 mins followed by reperfusion for 24 h.And the control group was only to be isolated cervical vessels,with no thread embolism inserted.The auditory brainstem response (ABR) was tested before operation and at 24 h post-operation respectively.The neurological deficits,ratio of infarct volume were evaluated.The pathological changes were observed by HE.Neurocyte apoptosis were observed by Tunel,and the AI were determined.The expression of GRP78,Caspase-12 were detected by immunohistochemistry and Western blotting.Results Compared with the sham group,the neurological function scores,the infarct volume of brain of I/R group increased significantly.The threshold of ABR elevated significantly and AI rised in I/R group.HE staining showed that the neurons in the sham group were arranged in order,the shape was normal,but in the I/R group the normal structure disappeared,the nuclei were condensed.The expression of GRP78,Caspase-12 protein were significantly up-regulated.All of the differences above have statistical significance(P＜0.05).Conclusion The mechanism of hearing loss after focal cerebral ischemia-reperfusion injury in rats is perhaps related to endoplamic reticulum stress,and GRP78,Caspase-12 participate in the process of neuron apoptosis on auditory cortex caused by ERS.关键词
脑缺血/内质网应激/缺血再灌注损伤/听性脑干反应Key words
endoplamic reticulum stress/apoptosis/ischemia-reperfusion injury/auditory brainstem response分类
医药卫生引用本文复制引用
吕哲,张颖,时美娟,孟晴,宋永周,路虹..内质网应激对大鼠局灶性脑缺血再灌注后听皮层损伤的作用[J].重庆医学,2018,47(11):1440-1443,1448,5.基金项目
河北省卫生和计划生育委员会医学科学研究重点课题(20160107). （20160107）
