重庆医学2018，Vol.47Issue(11)：1452-1456,5.DOI:10.3969/j.issn.1671-8348.2018.11.006
LBH589/来那度胺调控多发性骨髓瘤细胞中IRF4并促进凋亡反应的研究
LBH589/lenalidomide regulates, IRF4 and promotes apoptosis of multiple myeloma cells
唐思诗 1马丹 2成冰清 3余琨琳 1王季石 1彭焱3
作者信息
- 1. 贵州医科大学,贵阳550004
- 2. 南华大学附属怀化医院血液科 418000
- 3. 贵州医科大学附属医院血液科,贵阳550004
- 折叠
摘要
Abstract
Objective We aimed to investigate the expression of IRF4 and apoptosis of the histone deacetylase inhibitor LBH589 against MM cells in vitro,and study the mechanism of apoptosis when LBH589 alone or/and combined with lenalidomide in RPMI8226 cell so as to provide a new strategy for the treatment of multiple myeloma.Methods The cell viability on the growth of RPMI8226 cell were assessed by CCK8 assay.Apoptosis were measured by flow cytometry,The Grandpad software analyzes the statistical significance and evaluates the synergistic effect of the drug.The expression level of the related transcription factor and apoptotic gene protein were determined by western blot.The cell viability on the growth of RPMI8226-R cell were assessed by CCK8.Results LBH589 combined with lenalidomide have significant effect on inhibit cell proliferation and induce apoptosis in a dose-dependent manner.Apoptosis induced by LBH589/lenalidomide alone or combination was shown to involved PARP activation,decreased Bcl-2 and Bcl-xl expression.significantly down regulated transcriptional related factors of IRF4 and c-MYC expression compared with either agent alone.Conclusion LBH589 and lenalidomide alone or combination decrease the expression of transcription factor IRF4 and c-MYC,and have a significant synergistic effect,and highly expression of IRF in RPMI8226-R reduce proliferation inhibition.关键词
组蛋白去乙酰化酶抑制剂/来那度胺/多发性骨髓瘤/IRF4Key words
histone deacetylase inhibitor/lenlidomide/multiple myeloma/IRF4分类
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唐思诗,马丹,成冰清,余琨琳,王季石,彭焱..LBH589/来那度胺调控多发性骨髓瘤细胞中IRF4并促进凋亡反应的研究[J].重庆医学,2018,47(11):1452-1456,5.
