重庆医学2018，Vol.47Issue(12)：1572-1574,1579,4.DOI:10.3969/j.issn.1671-8348.2018.12.002
蒲公英甾醇对氧化损伤心肌细胞保护作用研究
Protective effects of Taraxasterol on oxidatively injured cardiomyocytes
摘要
Abstract
Objective To observe the protective effect and mechanism of taraxasterol on cardiomyocytes in oxidative injury model caused by ischemia-reperfusion (I/R).Methods Mouse cardiomyocytes (CSC cells) were used as the study objects and divided into the normal control group,I/R group,taraxasterol treating I/R group (5,10,30 μmol/L) and positive control group.The cell viability was measured by MTT.The expressions of Caspase-3 and Bcl-2 were detected by RT-PCR.The expressions of superoxide dismutase (SOD) and malondialdehyde (MAD) were detected by biochemical methods.The expression of ERK1/2 was detected by western blot.Results MTT assay showed that 30 μmol/L taraxasterol increased the cell viability of CSC cells injured by I/R (P＜0.05).The RT-PCR results showed that the expression of Caspase-3 mRNA was decreased with 10,30 μmol/L taraxasterol treatment,the difference was statistically significant when compared with I/R group (P＜0.05).The expression of Bcl-2 mRNA was increased with 30 μmol/L taraxasterol treatment,the difference was statistically significant when compared with the I/R group (P＜0.05).The biochemical method detection showed that 30 μmol/L taraxasterol induced SOD expression was increased and MAD expression was decreased,the difference was statistically significant when compared with the I/R group (P＜0.05).Western blot detection showed that 30 μmol/L taraxasterol treatment increased the ratio of p-ERK1/2 to t-ERK1/2 in injured CSC cells (P＜0.05).Conclusion Taraxasterol might inhibit ischemia-reperfusion caused cardiomyocyte oxidative injury by up-regulation of ERK1/2 expression.关键词
蒲公英/甾醇类/肌细胞,心脏/蒲公英甾醇/心肌缺血再灌注损伤/细胞外调节蛋白激酶1/2Key words
taraxacum mongolicum/sterols/myocytes, cardiac/taraxasterol/myocardial ischemia-reperfusion injury/ERK1/2分类
医药卫生引用本文复制引用
王娓娓,张红苗,王琳,鲍天昊..蒲公英甾醇对氧化损伤心肌细胞保护作用研究[J].重庆医学,2018,47(12):1572-1574,1579,4.基金项目
云南省教育厅资助性项目(2016ZZX093). （2016ZZX093）
