重庆医学2018，Vol.47Issue(12)：1575-1579,5.DOI:10.3969/j.issn.1671-8348.2018.12.003
Nodal及其受体在不同发育期小鼠组织器官中的表达
Expression of Nodal and its receptors in mouse tissues and organs at different ontogenetic stages
摘要
Abstract
Objective To investigate the expression of Nodal and its receptors in different tissues and organs of different development stages of mice.Methods Ten pairs(male and female each for a pair) of mice were divided into the four groups:3 pairs served as adult mice group,the rest 7 pairs were allowed to mate,among them 3 pregnant mice served as the fetal group,and 3 pregnant mice served as the neonatal group and 1 pregnant mouse served as filial group.The multiple tissues and organs such as brain,liver,kidney,heart,lung were selected from fetal,neonatal,filial and adult mice for preparing the protein samples.Western blot was performed to detect the expression of Nodal and its type Ⅰ receptors of ALK7 and ALK4 as well as auxiliary receptor Cripto-1.Results Only cerebrum,cerebellum,liver and kidney had Nodal express in the four different mouse ontogenetic stages,in which only liver and kidney simultaneously expressed Nodal and its receptor protein in the whole four ontogenetic stages.Besides,most tissues and organs of adult mice expressed Nodal and its receptor protein,which was significantly different from the fetal,neonatal and filial mice.Conclusion Nodal signaling might have a certain effect on the growth and development of mouse liver and kidney during the late development stage.关键词
Nodal信号/小鼠/个体发育/蛋白表达Key words
Nodal signaling/mice/ontogenesis/protein expression分类
生物科学引用本文复制引用
王轲,朱敏,顾洪,李念峰,胡湘麟,曾凡才..Nodal及其受体在不同发育期小鼠组织器官中的表达[J].重庆医学,2018,47(12):1575-1579,5.基金项目
四川省教育厅自然基金项目(110324) （110324）
国家级大学生创新创业训练计划项目(201410632008,201510632002) （201410632008,201510632002）
四川省科技厅-泸州市-泸州医学院联合专项基金(LY-100). （LY-100）
