重庆医学2018，Vol.47Issue(12)：1580-1582,3.DOI:10.3969/j.issn.1671-8348.2018.12.004
ZBTB20在SLE患者外周血B细胞中的表达和临床意义
Expression of zinc finger protein ZBTB20 in peripheral blood B cells in patients with SLE and its clinical significance
摘要
Abstract
Objective To study the expression of zinc finger and BTB domain containing 20 (ZBTB20) in peripheral blood B cells of the patients with systemic lupus erythematosus (SLE),and to investigate its clinical significance in SLE.Methods The ZBTB20 mRNA expression level in peripheral blood CD19+B cells of 36 cases of SLE (SLE group) and 30 healthy controls(control group) was detected by RT-PCR.The ZBTB20 protein level was detected by western blot;the proportion of B cell subset was measured by flow cytometry.Then the relationship between the ZBTB20 mRNA expression with B cells subset proportion change and its correlation with clinical indicators[anti-dsDNA antibody,immunoglobulin G (IgG),anti-nucleosome antibody (ANA) and anti-extractable nuclear antigen (ENA) antibody] in SLE patients were analyzed.Results The expression level of ZBTB20 mRNA in peripheral blood CD19+B cells of the SLE group was significantly higher than that of the control group (P＜0.05).The peripheral blood ZBTB20 protein level was higher than that of the control group (P＜0.05);the peripheral blood B cells subsets and CD19+ B cells proportion in the SLE patients were decreased,while the CD19-CD138+ plasmocytes/CD19+ B cells ratio and CD19-CD138+ plasmocytes proportion were significantly increased (P＜0.05).The expression level of ZBTB20 mRNA in B cells of SLE patients was positively correlated with the ratio of CD19-CD138+ plasmocytes/CD19+ B cells (P＜0.05).The expression of ZBTB20 mRNA was positively correlated with anti-ds-DNA antibody,ANA and anti-ENA antibody (P＜0.05).Conclusion ZBTB20 might participate in the pathogenesis of SLE possibly via promoting B cells differentiation.关键词
红斑狼疮,系统性/ZBTB20/锌指蛋白/B细胞Key words
lupus erythematosus, systemic/ZBTB20/zinc finger protein/B cells分类
医药卫生引用本文复制引用
裴露,王婷婷,朱峰,胡文坛,刘红春..ZBTB20在SLE患者外周血B细胞中的表达和临床意义[J].重庆医学,2018,47(12):1580-1582,3.基金项目
河南省科技攻关基金资助项目(172102310028) （172102310028）
河南省教育厅重点科技攻关项目(18A320007) （18A320007）
河南省卫计委医学科技攻关普通项目(201602042). （201602042）
