重庆医学2018，Vol.47Issue(12)：1583-1588,6.DOI:10.3969/j.issn.1671-8348.2018.12.005
HMGB-1联合MSCs移植对急性心肌梗死大鼠心脏功能影响的实验研究
Experiment of effects of HMGB-1 combined with MSCs transplantation on cardiac function in rats with acute myocardial infarction
摘要
Abstract
Objective To investigate the effects and mechanisms of HMGB-1 combined with MSCs transplantation on the heart function in rat with acute myocardial infarction.Methods A total of 144 male SD rats were divided into the healthy control group,model control group,MSCs transplantation group,HMGB-1 injection group,HMGB-1 injection+ MSCs transplantation group,HMGB-1 BoxA injection+MSCs transplantation group.On the 28th day after surgery,the heart function,myocardial pathological section,myocardial infarction area and new vessel density in infarction area were detected.Andthe level of related serum cytokines were measured on the 3rd,7th and 28th days after surgery.Results On the 28th day after surgery,left ventricular end diastolic dimension (LVDd) and left ventricular iiaternal diameter at end-systole (LVDs) in the HMGB-1 injection+ MSCs transplantation group were significantly decreased and the fractional shortening (FS) and ejection fraction (EF) value were significantly increased compared with the other five groups (P＜0.05);the infarction area in the HMGB-1 injection+MSCs transplantation group was significantly decreased and the new vessels number in the infarction area was significantly increased compared with the other model groups (P＜0.05).On the 3rd and 7th days after surgery,serum TLR4 and VEGF levels in the HMGB-1 injection+MSCs transplantation group were the highest (P＜0.05).On the 7th and 28th days after surgery,the levels of serum IL-6,NF-κB and TNF-α in the HMGB-1 injection+MSCs transplantation group were the lowest among all groups (P＜0,05).Conclusion HMGB-1 injection combined with MSCs transplantation treatment can effectively improve the prognosis of myocardial infarction.关键词
高迁移率族蛋白-1/骨髓间充质干细胞/心肌梗死/炎症因子/心功能Key words
high mobility group Box-1/mesenchymal stem cells/myocardial infarction/inflammatory cytokines/cardiac function分类
医药卫生引用本文复制引用
唐一锋,石蓓,王冬梅,沈长银..HMGB-1联合MSCs移植对急性心肌梗死大鼠心脏功能影响的实验研究[J].重庆医学,2018,47(12):1583-1588,6.基金项目
贵州省科技计划课题(黔科合SY字[2012]3109号). （黔科合SY字[2012]3109号）
