南方医科大学学报2018，Vol.38Issue(4)：390-394,5.DOI:10.3969/j.issn.1673-4254.2018.04.04
虫草素对人舌癌细胞凋亡、自噬的影响及其分子机制
Effect of cordycepin on apoptosis and autophagy of tongue cancer cells in vitro and the molecular mechanism
摘要
Abstract
Objective To study the effect of cordycepin on cell cycle, apoptosis and autophagy of human tongue cancer TCA-8113 cells and explore the mechanism of cordycepin for inhibiting the occurrence of tongue cancer.Methods CCK-8 method was used to assess the inhibitory effect of cordycepin on TCA-8113 cell proliferation in vitro.The cell cycle and cell apoptosis of TCA-8113 cells treated with different concentrations of cordycepin were analyzed using flow cytometry. The expressions of apoptosis-related genes caspase-3, caspase-9, Bcl-2, and Bax were examined using quantitative real-time PCR and Western blotting, and immunohistochemistry was used to detect the expressions of autophagy-related proteins LC-3β, P62, p-mTOR, and AMPK. Results CCK-8 assay showed that cordycepin significantly inhibited the proliferation of TCA-8113 cells in a concentration-dependent manner with an IC50of 3.548 mg/mL at 24 h and an IC50of 1.185 mg/mL at 48 h. Flow cytometric analysis showed that cordycepin caused cell cycle arrest at S phase and dose-dependently increased the apoptotic rate of TCA-8113 cells.Treatment of the cells with cordycepin enhanced the expressions of Bax,caspase-3 and caspase-9 at both the mRNA and protein levels and inhibited the expression of the antiapoptotic gene Bcl-2. Immunohistochemistry demonstrated that cordycepin promoted the expression of LC-3β and AMPK and inhibited the expression of P62 and p-mTOR. Conclusion Cordycepin inhibits the proliferation and induces apoptosis of HCT-116 cells through the mitochondrial pathway and induces autophagy via the AMPK/mTOR pathway.关键词
虫草素/自噬/凋亡/增殖Key words
cordycepin/autophagy/apoptosis/proliferation引用本文复制引用
郑庆委,高淑娴,吕杰,陈登宇,陈杰,李慧慧,管俊昌..虫草素对人舌癌细胞凋亡、自噬的影响及其分子机制[J].南方医科大学学报,2018,38(4):390-394,5.基金项目
国家自然科学基金(81571454) （81571454）
安徽省教育厅重点项目(KJ2017A222,KJ2016A461) （KJ2017A222,KJ2016A461）
蚌埠医学院科技发展基金(BYKF1705)Supported by National Natural Science Foundation of China(81571454). （BYKF1705）
