南方医科大学学报2018，Vol.38Issue(4)：402-408,7.DOI:10.3969/j.issn.1673-4254.2018.04.06
雌二醇显著上调骨质疏松大鼠成骨细胞内抗氧化酶的表达
Estradiol significantly increases the expression of antioxidant enzymes in osteoporotic rats and osteoblasts in vitro
摘要
Abstract
Objective To investigate the effect of estradiol on the expression of antioxidant enzymes in osteoblasts and its role in postmenopausal osteoporosis.Methods Rat models of osteoporosis established by ovariectomy were treated with estradiol for 3 months, and the changes in serum levels of reactive oxygen species (H2O2) and antioxidant enzymes (γ-GCS, GSH-ST and GSH-px)were detected.The effects of estradiol on the expression of γ-GCS mRNA and protein in osteoblast-like cells MC3T3-E1,MG63 and OB were examined with PCR and Western blotting.Using a mRNA microarray,we analyzed the changes in the expressions of 84 antioxidant enzymes in the osteoblast cell line MC3T3-E1 following estradiol treatment, and the enzymes with significant changes were verified by PCR.CCK-8 kit was used to evaluate the effect of estradiol and antioxidant NAC on the proliferation of MC3T3-E1 cells. Results Rat models of osteoporosis were successfully established with ovariectomy. The osteoporotic rats showed significantly increased serum level of reactive oxygen species (H2O2) and decreased levels of antioxidant enzymes. Estrogen treatment of the osteoporotic rats obviously reversed the phenotype of osteoporosis, lowered serum level of reactive oxygen species,and increased the level of γ-GCS.In MC3T3-E1,MG63 and OB cells,estradiol treatment significantly upregulated the expression levels of γ-GCS mRNA and protein. In MC3T3-E1 cells treated with estrogen, the mRNA chip identified 6 upregulated antioxidant enzymes (Gpx6, Gstk1, Nos2, Prdx2, Ngb and Ccs), and the results of PCR verified that estradiol upregulated Ccs and Ngb mRNAs in MC3T3-E1, MG63 and OB cells. Estradiol and antioxidant NAC obviously promoted the proliferation of MC3T3-E1 cells. Conclusion Estradiol significantly increases the expression of antioxidase γ-Gcs, Ccs and Ngb in osteoblasts in vitro. Postmenopausal osteoporosis is closely related with the increase of reactive oxygen species and the decrease of antioxidant levels. In osteoblasts, estrogen deficiency may increase the level of reactive oxygen species, decrease the level of antioxidant enzymes, activate the oxidative stress cascade, and consequently inhibit the proliferation of osteoblasts to aggravate the condition of osteoporosis.关键词
雌二醇/抗氧化酶/骨质疏松Key words
estradiol/antioxidant enzymes/osteoporosis引用本文复制引用
周雪娟,夏颖,赵岩岩,古文清,肖潇,白晓春,刘俊,李明..雌二醇显著上调骨质疏松大鼠成骨细胞内抗氧化酶的表达[J].南方医科大学学报,2018,38(4):402-408,7.基金项目
国家自然科学基金(31371186)Supported by National Natural Science Foundation of China(31371186). （31371186）
