南方医科大学学报2018，Vol.38Issue(4)：409-413,5.DOI:10.3969/j.issn.1673-4254.2018.04.07
氯喹通过miR-26b调控Mcl-1诱导人肝癌HepG2细胞凋亡
Chloroquine induces apoptosis of human hepatocellular carcinoma cells in vitro by miR-26b-mediated regulation of Mcl-1
摘要
Abstract
Objective To investigate the effect of chloroquine in inducing apoptosis of human hepatocellular carcinoma cells and explore the possible mechanism. Methods MTT assay and flow cytometry were used to evaluate chloroquine-induced growth inhibition and apoptosis in human hepatocellular carcinoma HepG2 cells,respectively.The ATP levels in chloroquine-treated cells were detected using an ATP assay kit. PCR and Western blotting were used to detect the expression levels of miR-26b and Mcl-1 in the cells, respectively. Results Chloroquine inhibited the proliferation of HepG2 cells in a time- and concentration-dependent manner.Treatments with 80 μmol/L chloroquine for 24,48,and 72 h induced survival rates of(71.59± 0.2)%,(45.40±0.5)%,and(26.34±1.4)% in the cells.Treatments with chloroquine at 40,80,and 160 μmol/L for 5 h resulted in obviously lowered intracellular ATP levels in the cells to 87.80%, 71.29%, and 38.02% of the control level, respectively.At 80 μmol/L,chloroquine significantly increased the expression of miR-26b and down-regulated the expression of Mcl-1 in HepG2 cells, and the application of the miR-26b inhibitor increased the cellular expression of Mcl-1. Conclusions Chloroquine can inhibit the cell proliferation, reduce ATP level and induce apoptosis in HepG2 cells possibly through miR-26b-mediated regulation of Mcl-1.关键词
肝癌细胞/氯喹/miR-26b/Mcl-1/凋亡Key words
hepatocellular carcinoma cells/chloroquine/miR-26b/Mcl-1/apoptosis引用本文复制引用
孙小锦,马琳艳,张梦晓,王颖,张配,蒋琛琛,刘浩..氯喹通过miR-26b调控Mcl-1诱导人肝癌HepG2细胞凋亡[J].南方医科大学学报,2018,38(4):409-413,5.基金项目
国家自然科学基金(81372899,8160131065) （81372899,8160131065）
蚌埠医学院自然科学基金项目(BYKY1641) （BYKY1641）
蚌埠医学院自然科学基金(BYKY1607ZD)Supported by National Natural Science Foundation of China(81372899,8160131065). （BYKY1607ZD）
