南方医科大学学报2018，Vol.38Issue(4)：414-420,7.DOI:10.3969/j.issn.1673-4254.2018.04.08
基于亚甲基蓝荧光特性研制的近红外荧光成像系统
Development of a near-infrared fluorescence imaging system based on fluorescence properties of methylene blue
摘要
Abstract
Objective To develop a near-infrared fluorescence imaging system based on the fluorescence properties of methylene blue. Methods According to the optical properties of methylene blue, we used a custom-made specific LED light source and an interference filter, a CCD camera and other relevant components to construct the near-infrared fluorescence imaging system. We tested the signal-to-background ratio (SBR) of this imaging system for detecting methylene blue under different experimental conditions and analyzed the SBR in urine samples collected from 15 Wistar rats with intravenous injection of methylene blue at the doses of 0, 1.4, 1.6, 1.8, or 2.0 0 mg/kg methylene blue. Results The SBR of this imaging system for detecting methylene blue was affected by the concentration of methylene blue and the distance from the sample(P<0.05).In the urine samples from Wistar rats,the SBR varied with the the injection dose,and the rats injected with 1.6 mg/kg methylene blue showed the highest SBR(8.71±0.20)in the urine(P<0.05).Conclusion This near-infrared fluorescence imaging system is useful for fluorescence detection of methylene blue and can be used for real-time recognition of ureters during abdominal surgery.关键词
输尿管/亚甲基蓝/近红外荧光成像系统Key words
ureter/methylene blue/near-infrared fluorescence imaging system引用本文复制引用
黄陆茂,杜佩妍,陈兰,张洒,周地福,陈春林,辛学刚..基于亚甲基蓝荧光特性研制的近红外荧光成像系统[J].南方医科大学学报,2018,38(4):414-420,7.基金项目
国家自然科学基金(61528102,61671229) （61528102,61671229）
广东省自然科学基金(2015A030313234) （2015A030313234）
广东省省级科技计划项目(2016A050502026) （2016A050502026）
科技开发培育计划项目(KJ20160519)Supported by National Natural Science Foundation of China(61528102,61671229). （KJ20160519）
