南方医科大学学报2018，Vol.38Issue(4)：421-427,7.DOI:10.3969/j.issn.1673-4254.2018.04.09
长链非编码RNA MALAT1促进皮肤鳞癌的发生和发展
Role of long noncoding RNA MALAT1 promotes the occurrence and progression of cutaneous squamous cell carcinoma
摘要
Abstract
Objective To investigate the role of long noncoding RNA MALAT1 in the occurrence and progression of cutaneous squamous cell carcinoma(CSCC).Methods Fifty-five tissue samples of CSCC and 10 normal epidermal tissues were collected for examination of the expression of MALAT1 using q-PCR and in situ hybridization. Human CSCC A431 cells were transfected with small interfering RNAs (siNC, siMALAT1-1, and siMALAT1-2) using Lipofectamine2000 to knock down MALAT1 gene, and the changes in the cell migration, invasion, mobility and proliferation were analyzed using Transwell assay,wound healing assay,and CCK-8 assay;the changes in the expressions of the related factors of epithelial-mesenchymal transition (EMT), including E-cadherin, vimentin, and β-catenin, were detected using qRT-PCR. Results Compared with normal tissues,CSCC tissues of different grades of differentiation all showed significantly increased expression of MALAT1(P<0.001).In A431 cells,MALAT1 knockdown with siRNAs resulted in significantly lowered cell proliferation(P<0.001),migration (P<0.01), invasion (P<0.01), and mobility (P<0.01). Knocking down MALAT1 gene also caused significantly increased expressions of E-cadherin and β-catenin (P<0.01) and lowered the expression of vimentin (P<0.01) in A431 cells. Conclusion The long noncoding RNA MALAT1 promotes the occurrence and progression of CSCC and can potentially serve as a therapeutic target in treatment of CSCC.关键词
LncRNA/MALAT1/皮肤鳞癌/上皮间质转化Key words
long noncoding RNA/MALAT1/cutaneous squamous cell carcinoma/epithelial-mesenchymal transition引用本文复制引用
李姗姗,周良,高琳,王颖慧,丁振华..长链非编码RNA MALAT1促进皮肤鳞癌的发生和发展[J].南方医科大学学报,2018,38(4):421-427,7.基金项目
国家自然科学基金(81573076,81472922,81673105)Supported by National Natural Science Foundation of China(81573076,81472922,81673105). （81573076,81472922,81673105）
