南方医科大学学报2018，Vol.38Issue(4)：477-482,6.DOI:10.3969/j.issn.1673-4254.2018.04.18
甘草次酸选择性抑制肝癌细胞的增殖
Glycyrrhetinic acid selectively inhibits proliferation of hepatocellular carcinoma cells in vitro
摘要
Abstract
Objective To investigate the selective inhibitory effect of glycyrrhetinic acid on 4 hepatocellular carcinoma (HCC) cells with different proliferation rates and explore the underlying mechanisms.Methods MTT method was used to detect the proliferation rates of 4 HCC cell lines, namely SMMC-7721, SK-HEP1, HEPG2 and HEP3B. Following treatment of the cells with glycyrrhetinic acid(5,10,20,30,40,and 60 μmol/L),the cell viability was analyzed using MTT assay and the expressions of total ERK protein, p-ERK protein and topoisomerase IIα were detected using Western blotting. Results Among the 4 cell lines,SMMC-7721 had the lowest and SK-HEP1 had the highest proliferation rate.Treatment with glycyrrhetinic acid for 48 h dose-dependently inhibited the proliferation of all the 4 cell lines in vitro and produced the strongest inhibitory effect in SMMC-7721 cells with the IC50of 28.04 μmol/L.The proliferation rate of the cells was positively correlated with the expression levels of p-ERK and topoisomerase IIα, which were the lowest in SMMC-7721 cells and the highest in SK-HEP1 cells. Treatment with 50 μmol/L glycyrrhetinic acid significantly down-regulated the expressions of p-ERK and topoisomerase IIα in the 4 HCC cell lines (P<0.05), while 25 μmol/L glycyrrhetinic acid significantly reduced the expression of topoisomerase IIα and p-ERK in SMMC-7721, HEPG2 and HEP3B cells (P<0.05) but not in SK-HEP1 cells. Conclusion Glycyrrhetinic acid can inhibit the proliferation of different HCC cells particularly in cells with a low proliferation rate. The inhibitory effect of glycyrrhetinic acid might be mediated by reducing the expressions of topoisomerase IIα and inhibiting the ERK pathway.关键词
甘草次酸/肝癌细胞/增殖速率/拓扑异构酶Ⅱα/ERK通路Key words
glycyrrhetinic acid/hepatocellular carcinoma/proliferation rate/topoisomerase IIα/ERK pathway引用本文复制引用
张韫琪,蔡云,刘媛,赵博欣,李国锋..甘草次酸选择性抑制肝癌细胞的增殖[J].南方医科大学学报,2018,38(4):477-482,6.基金项目
广东省自然科学基金(2015A030313281) （2015A030313281）
