福建农业学报2017，Vol.32Issue(11)：1173-1177,5.DOI:10.19303/j.issn.1008-0384.2017.011.001
利用CRISPR/CAS9技术定点编辑水稻花粉特异表达基因OsIPA
CRISPR/CAS9-mediated Editing for Pollen-specific OsIPA Genein Rice
摘要
Abstract
Male gamete development is an essential step in rice reproduction process,and male sterility is crucial for the utilization of rice heterosis.Since the anther or pollen specific genes in rice might play a pivotal role in the male gamete development,the functions of OsIPA were of great interest to the scientists.This study applied CRISPR/CAS9 to generate an OsIPA mutant for an investigation.Two sites in the 1st exon of OsIPA were selected as the targets.After sequencing,a total of 8 different types of mutant,including deletion,substitution and single nucleotide insertion,were found in 24T0 transgenic plants.关键词
水稻/CRISPR/Cas9/基因编辑/OsIPAKey words
rice/CRISPR/Cas9/gene editing/OsIPA分类
农业科技引用本文复制引用
杨绍华,陈惠妹,周淑芬..利用CRISPR/CAS9技术定点编辑水稻花粉特异表达基因OsIPA[J].福建农业学报,2017,32(11):1173-1177,5.基金项目
福建省科技计划项目——省属公益类科研院所基本科研专项(2014R1019-13) （2014R1019-13）
福建省科技计划重大专项(2015NZ0002-3) （2015NZ0002-3）
福建省农业科学院科技创新项目(2014QA-3) （2014QA-3）
