福建农业学报2017，Vol.32Issue(11)：1193-1196,4.DOI:10.19303/j.issn.1008-0384.2017.011.005
鸭巴泰病毒一步法RT-PCR检测方法的建立
Establishment of One-step RT-PCR for Batai Virus Detection in Ducks
摘要
Abstract
For a rapid detection of Batai virus (BATV) in ducks,a one-step reverse transcription polymerase chain reaction (RT-PCR) assay was developed based on the conserved region of the M gene in BATV.With the optimized reaction conditions,this assay could specifically amplify a 480 bp fragment from the M gene.There were no similar gene amplifications on 5 other pathogens found in ducks,such as avian Tembusu virus and duck hepatitis virus.The testing sensitivity of the method was 1 × 101.1 TCID50 with a high reproducibility.Consequently,this newly developed methodology seemed to be highly specific and sensitive for BATV detection,and could be applied for rapid and early diagnosis as well as epidemiological investigations on BATV.关键词
巴泰病毒/鸭/RT-PCRKey words
batai virus/duck/RT-PCR分类
农业科技引用本文复制引用
傅光华,陈翠腾,傅秋玲,刘荣昌,程龙飞,施少华,万春和,陈红梅,黄瑜..鸭巴泰病毒一步法RT-PCR检测方法的建立[J].福建农业学报,2017,32(11):1193-1196,4.基金项目
现代农业产业技术体系建设专项(CARS-42) （CARS-42）
福建省科技计划项目——省属公益类科研院所基本科研专项(2015R1023-3、2017R1023-16) （2015R1023-3、2017R1023-16）
福建省农业科学院青年英才计划项目(YC2015-13) （YC2015-13）
福建省农业科学院科技扶贫项目(2017-4) （2017-4）
