福建农业学报2017，Vol.32Issue(11)：1201-1206,6.DOI:10.19303/j.issn.1008-0384.2017.011.007
白茶实时荧光定量PCR分析中内参基因的筛选与验证
Selection and Validation of Reference Genes for Real-time Florescence Quantitative PCR Analysis on Gene Expression of White Tea
摘要
Abstract
To select appropriate reference genes to be used in the real-time florescence quantitative PCR analysis for studying the gene expression in of white tea leaves during withering,the software of GeNorm,NormFinder,and BestKeeper were applied.Three stable reference genes,β-Actin,GAPDH and RUBP,were identified.Among them,RUBP was found most adequate for comparing the expressions at various stages of withering,while β-Actin for differentiating them in leaves from different parts of a tea plant.The combination of GAPDH+RUBP was determined as choice reference genes for the analysis.关键词
白茶/萎凋/内参基因/表达稳定性/荧光定量PCRKey words
white tea/withering/reference genes/stability/real-time fluorescence quantitative PCR分类
农业科技引用本文复制引用
陈静,郑知临,林浥,曹红利,陈笛,陈桂信,叶乃兴..白茶实时荧光定量PCR分析中内参基因的筛选与验证[J].福建农业学报,2017,32(11):1201-1206,6.基金项目
国家自然科学基金项目(31270735) （31270735）
福建农林大学科技创新专项(CXZX2016117、CXZX2017313) （CXZX2016117、CXZX2017313）
