福建农业学报2017，Vol.32Issue(11)：1213-1217,5.DOI:10.19303/j.issn.1008-0384.2017.011.009
猕猴桃查尔酮合成酶基因CHS密码子偏好性分析
Codon Usage Bias of Chalcone Synthase Gene CHS in Kiwifruit
摘要
Abstract
To investage codon usage characteristic of chalcone synthase gene of kiwifruit,CodonW,SPSS,MEGA software and EMBOSS online program were used to analyse codon usgae bias (CUB) of CHS,and phylogenetic tree of CHS between monocotyledon and dicotyledon was constructed in this paper.The results showed that,the effective number of codons (ENc),total GC content (GC) and GC content on the 3rd site (GC3s) was 57.21,0.533 and 0.607,respectively.The number of condons,the number of relative synonymous codon usage (RSCU) of which was more than 1.0,was 30,but no codons was with strongly CUB.The cluster tree of CHS based on CUB was closer to the real category system than it did by sequence similarity.According to codon usage frequency,the CUB between CHS of kiwifruit and Arabidopsis thaliana,Escherichia coli were more similar.These results indicated that,the CUB level of CHS was low in kiwifruit,but it was bias toward GC rich codons and synonymous codons with G or C at 3rd codon position.And CHS in monocotyledons and dicotyledons had the distinctive CUB rules.In addition,Arabidopsis thaliana and Escherichia coli migth be the best receptors for genetic transformation and heterologous expression of CHS in kiwifruit.关键词
猕猴桃/查尔酮合成酶/密码子使用偏好性/聚类分析Key words
Kiwifruit/chalcone synthase/codon usage bias/cluster analysis分类
农业科技引用本文复制引用
陈义挺,赖瑞联,冯新,高敏霞,张长和,吴如健..猕猴桃查尔酮合成酶基因CHS密码子偏好性分析[J].福建农业学报,2017,32(11):1213-1217,5.基金项目
福建省自然科学基金项目(2017J01044) （2017J01044）
福建省科技计划项目——省属公益类科研院所基本科研专项(2015R1014-3) （2015R1014-3）
福建省农业科学院项目(A2016-2) （A2016-2）
福建省农业科学院青年创新团队项目(STIT2017-3-6) （STIT2017-3-6）
