福建农业学报2017，Vol.32Issue(11)：1224-1227,4.DOI:10.19303/j.issn.1008-0384.2017.011.011
淡水砂梨离体快繁技术研究
In Vitro Propagation of Dan-Shui Pear
摘要
Abstract
To establish an in vitro culture system for propagating Dan-Shui pear,Xiangshui pear was used on Murashige and Skoog medium (MS) as the basal medium for the experimentation.Conditions of the tissue culture,such as hormone combination and concentration on explant induction and proliferation culture,as well as hormone types and concentrations on rooting culture,were studied.The optimal combination for the axillary bud induction was found to be MS+ 6-BA1.0mg· L 1+NAA0.1mg· L-1 that yielded a germination rate of 71.7 ％.For the bud proliferation,it was MS+6-BA 3.0 mg· L-1 + NAA 0.20 mg · L-1 to deliver a multiplication coefficient of 3.2.For the optimal rooting performance,the combination of 1/2 MS+IBA 0.3 mg · L-1 to result in a rooting rate of 63.3％ was selected.Thus,addition of the growth regulators to MS medium benefitted the in vitro propagation of Dan-Shui pear.关键词
淡水砂梨/离体快繁/培养基/增殖Key words
dan-Shui pear/in vitro propagation/culture medium/multiplication分类
农业科技引用本文复制引用
曾令达,李彩华,翟秋艳..淡水砂梨离体快繁技术研究[J].福建农业学报,2017,32(11):1224-1227,4.基金项目
国家自然科学基金(31272117) （31272117）
广东省科技计划项目(2013B020304010) （2013B020304010）
惠州市科技计划项目(2013B040009001) （2013B040009001）
