福建农业学报2017，Vol.32Issue(11)：1257-1262,6.DOI:10.19303/j.issn.1008-0384.2017.011.017
不同蝉花菌株的分子鉴定及产透明质酸比较
Molecular Identification and Productivity on Hyaluronic Acid in Submerged Culture of Different Cordyceps cicadae Strains
摘要
Abstract
This study aimed to identify 4 Cordyceps cicadae strains and compare their productivities on hyaluronic acid (HA) in a liquid culture.The molecular identification for CC1502,CC1504,CC1601 and CC1602 was conducted based on their internal transcribed spacer (ITS) sequences and using the randomly amplified polymorphic DNA (RAPD) analysis.The results indicated that there was no difference on ITS sequences among these fungal strains,and that the RAPD patterns were amplified by the primer,AP-A20,AP-D18 or AP-H18.As shown by the dendrogram analysis with UPGMA method,CC1504 had a low genetic similarity with any of the other strains,and CC1601 related closely to CC1602.The liquid fermentation of the strains showed different levels of HA productivity between CC1502 and CC1504,and glucose and peptone were the choice carbon and nitrogen sources.The maximum HA production was up to 2.45 mg · mL-1 generated by CC1502,which,therefore,was considered a candidate for industrial application in the future.关键词
蝉花/随机扩增多态性/液体发酵/透明质酸Key words
Cordyceps cicadae/randomly amplified polymorphic DNA/liquid fermentation/hyaluronic acid分类
生物科学引用本文复制引用
龙良鲲,黄曙惠,石子玉,林群英,李传华,丁少军..不同蝉花菌株的分子鉴定及产透明质酸比较[J].福建农业学报,2017,32(11):1257-1262,6.基金项目
江苏省高校自然科学基金(15KJB220003) （15KJB220003）
江苏省高等学校大学生创新创业训练计划项目(201610298104X) （201610298104X）
上海市科技兴农重点攻关项目[沪农科攻字(2014)第7-1-6号] （2014）
