广西科学2018，Vol.25Issue(1)：63-67,5.DOI:10.13656/j.cnki.gxkx.20180206.004
基于新型核酸适配体-荧光分子检测探针的石斑鱼虹彩病毒病快速诊断
Establishment and Characterization of Novel Specific Aptamer-based Fluorescent Molecular Probe for Rapid Diagnosis of Grouper Iridovirus Diseases
摘要
Abstract
[Objective]Grouper iridovirus is the major virus pathogen in South China,and causes high mortality to marine cultured grouper,which seriously threatens the healthy and sustain able development for grouper marioulture.Then effective detective techniques for the rapid diagnosis of SGIV infection are urgently needed.[Methods] Based on the nucleotide aptamer (Q2),the rapid detection and diagnosis technology of SGIV disease was systematically studied and a new Aptamer Q2-based fluorescent molecular probe (Q2-AFMP) was developed.The specificity.,sensitivity and stability of Q2-AFMP indetecting SGIV infection were analyzed.[Re-sults]Aptamer Q2-Based fluorescent molecular probe(Q2-AFMP)could detect SGIV infection with high specificity,sensitivity and stability.[Conclusion]The novel fluorescent molecular probe(Q2-AFMP)holds great potentials for rapid early diagnosis of pathogen SGIV infection in grouper mariculture in South China.关键词
石斑鱼虹彩病毒/核酸适配体-荧光分子探针/高特异性和灵敏性/快速诊断Key words
Singapore grouper iridovirus/Q2-AFMP/high specificity and sensitivity/rapid early diagnosis分类
农业科技引用本文复制引用
李鹏飞,余庆,李菲,覃仙玲,董德信,陈宪云,牙韩争,柯珂,秦启伟..基于新型核酸适配体-荧光分子检测探针的石斑鱼虹彩病毒病快速诊断[J].广西科学,2018,25(1):63-67,5.基金项目
国家自然科学基金项目(41706145),广西自然科学基金项目(2017GXNSFBA198176)和广西科学院基本科研业务费项目(2017YJJ23002)资助. （41706145）
