广西科学2018，Vol.25Issue(1)：68-73,6.DOI:10.13656/j.cnki.gxkx.20180208.001
广西北部湾大宗海水养殖鱼类卵形鲳鲹感染溶藻弧菌及其致病性研究
Isolation, Identification and Pathogenicity of Vibrio alginolyticus from Marine Cultured Trachinotus ovatus in Beibu Gulf, Guangxi
摘要
Abstract
[Objective]The Trachinotus ovatus cultured in offshore cages in Qinzhou Bay of Guangxi Province showed typical symptoms of bacterial diseases in 2016,including skin ulceration,subcutaneous bleeding and visceral lesions.By isolating,identifying and preserving the pathogenic bacteria in the diseased T.ovatus,it established the foundation for the follow-up research on the rapid detection technology and prevention and control technology of pathogenic bacteria,as well as controlling the outbreak and epidemic of related pathogenic bacteria in the marine culture.[Methods]Four suspected pathogen strains were isolated from the ulcer lesions,kidney and liver of diseased T.ovatus by using LB and TCBS plates.Biological tests were conducted to check the conventional morphological characteristics and physiological and biochemical reactions.Molecular biology identification of the suspected pathogenic strains was performed by 16S rDNA gene sequencing.The pathogenicity of pathogenic bacteria was carried out at the level of cells and fish,and the re-inoculation of artificial infection was carried out simultaneously.[Results]Four strains of suspected pathogens (TOQZ01,TOQZ02,TOQZ03,TOQZ04) were isolated and these four strains were identified as Vibrio alginolyticus based on their phenotypes,molecular biological characteristics and evolutionary relationships.The cytotoxicity and regression tests confirmed that the isolated V.alginolyticus were the pathogen,which caused the bacterial diseases in T.ovatus.[Conclusion] Vibrio alginolyticus is one of the main pathogenic bacteria that cause bacterial fish disease in marine culture fish in the coastal areas of Guangxi.In the future,Vibrio alginolyticus isolated from this study will be used to carry out rapid disease detection technology and prevention and control technology and other key technologies of modern seawater eco-health farming in order to realize the rapid diagnosis,real-time monitoring and effective prevention of Vibrio alginolyticus in marine culture of Guangxi.关键词
卵形鲳鲹/溶藻弧菌/细胞毒性/防控建议Key words
Trachinotus ovatus/Vibrio alginolyticus/cytotoxicity/suggestion for prevention and control分类
农业科技引用本文复制引用
余庆,李菲,王一兵,覃仙玲,陈宪云,吴柳莹,李鹏飞..广西北部湾大宗海水养殖鱼类卵形鲳鲹感染溶藻弧菌及其致病性研究[J].广西科学,2018,25(1):68-73,6.基金项目
广西自然科学基金项目(2017GXNSFBA198176)和广西科学院基本科研业务费项目(2017YJJ23002)资助. （2017GXNSFBA198176）
