广西科学2018，Vol.25Issue(1)：74-79,6.DOI:10.13656/j.cnki.gxkx.20180206.001
广西卵形鲳鲹小脑来源细胞系的建立及特征分析
Establishment and Characterization of a Novel Cell Line from Cerebellum of Golden Pompano (Trachinotus ovatus) Cultured in Guangxi
摘要
Abstract
[Objective]Golden pompano (Trachin otus ovatus) is a commercially important marine fish species in Guangxi,but in recent years with the expansion of breeding scale,the frequent outbreaks of pathogens caused by various diseases and infections have caused great losses to the aquaculture industry of Trachinotus ovatus in Guangxi.The establishment of a cell line from cerebellum of Trachinotus ovatus (TOCC) would be conducive to the isolation and identifica-tion of viral pathogens in Guangxi oval salamander breeding and the pathogenic molecular mechanism of disease infection,as well as the dynamic monitoring of environmental pollution and changes.[Methods] The primary TOCC cells were cultured by trypsinization,and then subcultured after reaching 100％ confluence.18S rDNA gene sequence analysis was applied to identify the origin of TOCC.We further evaluated TOCC cells' characteristics of growth,chromosome,and toxicity of bacterial extracellular products on TOCC cells.[Results]TOCC cells were fibroblast-like cells,the 18S rDNA gene sequence analysis showed that it derived from Trachinotus ovatus.Chromosome karyotype analysis found that TOCC had diploid features,the number of characteristic chromosomes was 62.The growth rates of TOCC cell were different in different culture conditions.The optimal culture temperature for TOCC cells growth was 28℃.ECPs exhibited toxicity to TOCC cells.[Conclusion]A novel cell line derived from the cerebellum tissue of Trachinotus ovatus (TOCC) was successfully established in this study.Cytotoxicity results of bacterial extracellular products to TOCC showed that within a certain range the cell line could replace the in vivo detection and realize in vitro cell level rapid detection of the disease infection and environmental pollutants in Guangxi marine culture and provide a technical support for the effective prevention of aquaculture disease.关键词
卵形鲳鲹/小脑来源细胞系/细胞特征/细胞霉性Key words
Golden pompano (Trachinotus ovatus)/cerebellum cell line/cell characteristics/cytotoxicity分类
农业科技引用本文复制引用
余庆,李菲,覃仙玲,陈宪云,董德信,牙韩争..广西卵形鲳鲹小脑来源细胞系的建立及特征分析[J].广西科学,2018,25(1):74-79,6.基金项目
广西自然科学基金项目(2017GXNSFBA198176)和广西科学院基本科研业务费项目(2017YJJ23002)资助. （2017GXNSFBA198176）
