广西科学2018，Vol.25Issue(1)：80-86,7.DOI:10.13656/j.cnki.gxkx.20180123.001
球形棕囊藻北部湾株对不同形态磷源的利用及碱性磷酸酶特性研究
Study on the Utilization of Different Phosphorus and Alkaline Phosphatase Characteristics of Phaeocystis globosa Cultivated from Beibu Gulf
摘要
Abstract
[Objective]To explore the differences of Phaeocystis globosa BBW PG-01 from Beibu Gulf in utilizing different phosphorus and its characteristic of alkaline phosphatase(AP) can reveal the important role that DOP played when the algae bloom occurs.[Methods]Under laboratory culture condition and taking KH2PO4 (PO3-4),RNA,Glucose 6 phosphate(G-6-P),adenosine triphosphate (ATP) and lecithin (LEC) as phosphorus sources,the growth characteristics and Alkaline phosphatase activity (APA) of BBW PG-01 in these five different phosphorus sources were compared.Combining the inorganic phosphorus absorption kinetics and alkaline phosphatase kinetics under phosphorus starvation,the competitive mechanism of inorganic phosphorus and organic phosphorus was analyzed.[Results]It was found that five morphological phosphorus sources could be utilized by BBW PG-01.RNA was the optimal source of phosphorus for its growth,however,with macro-molecular organic phosphorus LEC as the phosphorus source,the growth was the worst.The maximum cell density for these two sources was 4.40× 108 cells/L and 2.39× 108 cells/L,hydrolysis of macromolecules DOP might require higher enzyme activity.Kinetic studies indicated that BBW PG-01 had the ability to compete for phosphorus sources to sustain growth with high affinity in the deficiency of phosphorus.[Conclusion]Under the action of AP,Phaeocystis globosa from Beibu Gulf could grow with many kinds of DOPs and had strong competitive ability to both DIP and DOP under low phosphorus conditions.DOP in the ocean might be an important phosphorus source for the outbreak and maintenance of Phaeocystis globosa red tide.关键词
球形棕囊藻/北部湾/有机磷/碱性磷酸酶Key words
Phaeocystis globosa/Beibu Gulf/organic phosphorus/alkaline phosphatase分类
生物科学引用本文复制引用
覃仙玲,陈波,赖俊翔,陆家昌,牙韩争..球形棕囊藻北部湾株对不同形态磷源的利用及碱性磷酸酶特性研究[J].广西科学,2018,25(1):80-86,7.基金项目
国家自然科学基金项目(41576024),广西重点研发计划项目(桂科AB16380282)和广西科学院基本科研业务费项目(15YJ22HY07)资助. （41576024）
