广西科学2018，Vol.25Issue(1)：87-93,99,8.DOI:10.13656/j.cnki.gxkx.20180115.001
海绵Pseudoceratina sp.共栖细菌多样性及其抑制甘蔗鞭黑粉菌活性研究
Diversity of Sponge-Derived Bacteria Isolated from Pseudoceratina sp.against Sporisorium scitamineum in vitro
摘要
Abstract
[Objective]The purpose of this study is to investigate the diversity of sponge-derived bacteria of Pseudoceratina sp.near the sea area of Xieyang Island in Guangxi and to screen for the active substances that inhibit the growth of Sporisorium scitamineam and lay the foundation for the discovery of potential bacterial new species and the development of agricultural biochemical fertilizer.[Methods]Using dilution coating method the sponge-derived bacteria were isolated from Pseudoceratina sp.through six different isolation media.The bacterial morphological observation was used to remove the redundancy.The 16S rRNA gene sequence of the strain was amplified by PCR,and the species diversity was analyzed by constructing a phylogenetic tree.The Oxford Cup method was used to the screening experiment of inhibiting the activity of Sporisorium scitamineam.[Results]54 strains of cultivable bacteria were isolated from Pseudoceratina sp.,and after 16S rRNA gene sequence comparison,24 strains of bacteria were obtained,belonging to 13 families and 14 genera.Among them,the highest similarity rate of strain BGMRC 2118 to the 16S rRNA gene sequence of the published effective strain was 96.46％,which might be a potential new species.Anti-bacterial screening experiments showed that the crude fermentation extracts of 8 strains had a strong inhibition effect against S.scitamineum.[Conclusion]The sponge-derived bacteria of Pseudoceratina sp.near the sea area of Xieyang Island in Guangxi had high genetic diversity,rich in new species resources and rich in bioactive strains.关键词
海绵/共附生细菌/物种多样性/甘蔗鞭黑粉菌/抑菌活性Key words
Pseudoceratina sp/sponge-derived bacteria/genetic diversity/Sporisorium scitamineum/antibacterial activities分类
生物科学引用本文复制引用
李菲,黄庶识,王伟,江蕾,米顺利,余炼..海绵Pseudoceratina sp.共栖细菌多样性及其抑制甘蔗鞭黑粉菌活性研究[J].广西科学,2018,25(1):87-93,99,8.基金项目
广西科技计划项目(AD17129019),广西青年科学基金项目(2016GXNSFBA380095)和广西科技计划项目(AB16380071)资助. （AD17129019）
