海南医学院学报2018，Vol.24Issue(7)：789-792,4.DOI:10.13210/j.cnki.jhmu.20180223.015
DNA倍体检测对宫颈癌病灶中癌细胞增殖、侵袭活性的评估价值研究
The value of DNA ploid detection for the evaluation of cancer cell proliferation and invasion activity in cervical cancer
摘要
Abstract
Objective:To study the value of DNA ploid detection for the evaluation of cancer cell proliferation and inva-sion activity in cervical cancer.Methods:Patients with cervical cancer who underwent surgical resection in Yan'an University Affiliated Hospital between March 2015 and May 2017 were selected as cervical cancer group of the research,and patients with benign lesions who underwent hysterectomy in Yan'an University Affiliated Hospital during the same period were selected as the control group of the research.Before operation,cervical exfoliated cytology was taken for DNA ploid detection;before op-eration,the cervical tissue was taken to determine the expression of proliferation genes and invasion genes.Results:The num-ber of exfoliated cells with abnormal DNA ploid in cervical cancer group was significantly higher than that in control group;Id-1,Ki-67,TET1,S6K1,CatL and ILK mRNA expression in cervical cancer tissue of cervical cancer group were significantly higher than those of control group whereas HSG,MCPH1,p16,TIMP1 and TIMP2 mRNA expression were significantly low-er than those of control group;Id-1,Ki-67,TET1,S6K1,CatL and ILK mRNA expression in cervical cancer tissue with ≥3 exfoliated cells with abnormal DNA ploid were significantly higher than those in cervical cancer tissue with 1-2 exfoliated cells with abnormal DNA ploid whereas HSG,MCPH1,p16,TIMP1 and TIMP2 mRNA expression were significantly lower than those in cervical cancer tissue with 1-2 exfoliated cells with abnormal DNA ploid.Conclusion:DNA ploid detection can be used to evaluate the proliferation and invasion of cancer cells in cervical cancer.关键词
宫颈癌/DNA倍体/增殖/侵袭Key words
Cervical cancer/DNA ploid/Proliferation/Invasion分类
医药卫生引用本文复制引用
张云清,尹香利,韩慧慧..DNA倍体检测对宫颈癌病灶中癌细胞增殖、侵袭活性的评估价值研究[J].海南医学院学报,2018,24(7):789-792,4.基金项目
延安市科学技术研究发展计划项目(2017KS-11-02)This study was supported by Science and Technology Research and Development Project of Yan'an City(Grant No.2017KS-11-02)． （2017KS-11-02）
