核农学报2018，Vol.32Issue(5)：840-847,8.DOI:10.11869/j.issn.100-8551.2018.05.0840
大豆芽黄新突变体vl-1的光合特性与基因定位
Identification and Fine Mapping of A New Virescent Mutant vl-1 in Soybean
摘要
Abstract
To discover new gene resources of leaf color for studying the mechanism of chlorophyll synthesis and photosynthesis,the soybean seeds of variety Nannong 99-6 were treated by60 Co-γ rays irradiation,and a novel virescent mutant vl-1 with emerging yellowleaveswas identifiedfrom M3 generation.The morphology,chloroplast structure,pigment content and photosynthetic characteristics of mutant vl-1 were investigated,and the inheritance and gene mapping were also studied.Compared with the wild type,the young leaves of mutant vl-1plants showed significant reduction of photosynthetic pigments,and the structure of chloroplast displayed fuzzier grana,looser stroma lamella and more prolamellar body.The mature leaves of mutant vl-1 turned green and there were no significant difference in the photosynthetic pigment content,net photosynthetic rate,stomatal conductance and transpiration rate between mutant vl-1 and the wild type,but the intercellular CO2 concentration in the mutant was significantly lower than that in the wild type.Genetic analysis suggested that the mutational trait was controlled by a single recessive gene designated as vl1.Using virescent individuals from the F2 population (KF1 × vl-1),the target locus vl1 was finally mapped in a 628 kb region on the top of chromosome 8 between SSR marker BARCSOYSSR_08_0037 and BARCSOYSSR_08_0073,containing 76 annotation genes.This study can make a well foundation for further research on genetic base of leaf color in soybean.关键词
大豆/芽黄突变体/光合特性/遗传/基因定位Key words
soybean/virescent mutant/photosynthetic characteristics/inheritance/gene mapping引用本文复制引用
孔可可,许孟歌,刘美凤,孔杰杰,盖钧镒,赵团结..大豆芽黄新突变体vl-1的光合特性与基因定位[J].核农学报,2018,32(5):840-847,8.基金项目
国家重点研发计划课题(2016YFD0101500、2016YFD0101504),国家自然科学基金(31271750、31571691),江苏省现代作物生产协同创新中心项目(JCIC-MCP) （2016YFD0101500、2016YFD0101504）
