核农学报2018，Vol.32Issue(5)：848-855,8.DOI:10.11869/j.issn.100-8551.2018.05.0848
基于高通量测序的青花菜早期发育小孢子转录组分析与基因功能注释
Transcriptome Analysis and Gene Function Annotation of Early Developmental Broccoli Microspores Based on High-throughput Sequencing Technology
摘要
Abstract
In order to investigate the molecular biological mechanisms of broccoli microspore embryogenesis,the Illumina HiSeqTM2000 platform was utilized to analyze and annotate transcriptomes of the microspores,cultured at 32.5℃ for 17 h and 24 h and at 25℃ for 0,1,6 d in this research.The results showed that a total of 174324 Unigenes were produced,among which 144 194 were annotated in GO、COG、KEGG、NR、Swissprot and Pfam database.The analysis of gene ontology function enrichment revealed that some important biological processes related to microspore embryogenesis,such as cell part,cell and organelle,binding and catalytic activity,metabolic process,cellular process and single-organism process,etc,got significantly enriched.The frequencies of R、L、K、T and O were much higher than others in COG function classification.The results of KEGG pathways enrichment showed that differentially expressed genes produced from microspores after heat shock tended to cluster into the pathway of protein processing in endoplasmic reticulum,the pathways of plant-pathogen interaction,plant hormone signal transduction and spiceosome,etc.The results laid a good foundation for further study on the physiological and biochemical and molecular mechanisms of broccoli microspore embryogenesis.关键词
小孢子胚胎发育/差异表达基因/转录组/基因功能注释Key words
microspore embryogenesis/differential expressed gene/transcriptome/gene function annotation引用本文复制引用
张振超,姚悦梅,毛忠良,孙国胜,秦文斌,戴忠良..基于高通量测序的青花菜早期发育小孢子转录组分析与基因功能注释[J].核农学报,2018,32(5):848-855,8.基金项目
国家自然科学基金-青年基金(31401871) （31401871）
