核农学报2018，Vol.32Issue(5)：864-874,11.DOI:10.11869/j.issn.100-8551.2018.05.0864
玉米NAC转录因子基因ZmNAM的克隆及表达分析
Cloning and Expression Analysis of Transcription Factor Gene ZmNAM in Maize (Zea mays L.)
摘要
Abstract
ZmNAM is a candidate gene identified in the previous study.To investigate its function in maize,ZmNAM was isolated from the sweet corn line "1132" and its variant line "704".Bioinformatics analysis showed that the cDNA of ZmNAM was 1548 bp and contained a 1302 bp open reading frame (ORF) encoding a hydrophilic protein with 433 amino acid residues.The ZmNAM protein had a conserved NAC domain in its N-terminal and was also predicted to locate in nucleus and contain nine glycosylation sites as well as 35 phosphorylation sites.Compared with inbreed line 1132,there were differences in nucleotide sequence of non-coding regions and the transcriptional actiration domain of Cterminal in coding region in variant line,which may lead to variation of multiple amino acids.Phylogenetic analysis revealed that ZmNAM was a member of NAC transcription factor family belonging to VND subgroup and had a closest genetic relationship with AtVND3 in Arabidopsis.The real-time PCR showed that expression level of ZmNAM in root was higher than that in other organs of maize in different development stages.It suggests that ZmNAM may play an important role in regulating the growth and development of root in maize.Furthermore,the expression level of ZmNAM in variation line 704 was higher than that in inbred line 1132 in most development stages and development organs,especially in root.These results provide a scientific evidence for further elucidating the role of ZmNAM during development of maize.关键词
玉米/NAC转录因子/基因克隆/荧光定量PCRKey words
maize/NAC transcription factor/gene cloning/RT-qPCR引用本文复制引用
林婕,于永涛,马三梅,胡建广..玉米NAC转录因子基因ZmNAM的克隆及表达分析[J].核农学报,2018,32(5):864-874,11.基金项目
广东省自然科学基金项目(2014A030313574),广东省科技计划项目(2015A020209063) （2014A030313574）
