核农学报2018，Vol.32Issue(5)：875-882,8.DOI:10.11869/j.issn.100-8551.2018.05.0875
窄冠型杨树杂交子代SRAP鉴定及其遗传变异研究
Identification and Genetic Variation Analysis of Narrow Crown Populus Hybrid Progeny by SRAP
摘要
Abstract
To identify the authenticity of hybrid progeny and elucide the relationship of offsping population in Popular,the technology of sequence related amplified polymorphism (SRAP) was used to identify the genetic variations in 7 hybrid offspring of narrow-crowned poplar populations in this study.High primers of 8 pairs of which amplify stable main bands with clear were screened from 110 pairs of SRAP primers.A total of 126 bands were amplified from 7 hybrid progenies,including 118 polymorphic bands and the percentage of polymorphic bands (PPB) was 93.65％.Analysis of genetic diversity by PopGen 32 software for 7 hybrid combinations showed that the genetic diversity of the hybrid progeny in the Populus nigra Linn was lower than in Populus alba.The genetic diversity of hybrid offspring which obtained from Populus alba (female) was more abundant than Populus nigra Linn (female).The correlation between the genetic distance and growth of the offspring,morphological and textural properities in 4 hybrids showed that the genetic distance between parents was positively correlated with the seedling height and ground diameter and negatively correlated with the branching angle.70 hybrid progeny have been proved to be the true hybrids which showed the male parent feature band.This research provides important theoretical basis and technical reference for screening the genetic diversity of poplar hybrids and early identification of hybrid progeny.关键词
窄冠型杨树/子代鉴定/SRAP分子标记/遗传多样性Key words
narrow crown Poplar/progeny identification/SRAP molecular marker/genetic diversity引用本文复制引用
田彦挺,李际红,王锦楠,许东,王艺玮,邢世岩..窄冠型杨树杂交子代SRAP鉴定及其遗传变异研究[J].核农学报,2018,32(5):875-882,8.基金项目
山东省农业良种工程项目(2013-2016),山东省自然基金项目(ZR2013CM020),国家高技术研究发展计划(863计划)(2013AA102703) （2013-2016）
