核农学报2018，Vol.32Issue(5)：883-891,9.DOI:10.11869/j.issn.100-8551.2018.05.0883
大黄鱼FST基因SNP筛选及与生长性状相关性分析
SNP Detection of FST Gene and Its Association With Growth Traits in Larimichthys crocea
摘要
Abstract
In order to explore the correlation between single nucleotide polymorphism (SNP) of FST gene and growth traits of Larimichthys crocea,an important marine-cultivated fish in China,SNP sites were screened and identified by direct sequencing and high resolution melt (HRM) in this study.The results showed that a total of 19 SNP sites were obtained,among which 2 in exon 1,8 in exon 2,7 in exon 3,1 in exon 4 and 1 in exon 5.However,no SNP site was obtained in the proximal promoter region.At the g.2169A ＞ C site located in exon 4,genotype frequencies of two genotypes AA and CC were 0.968 and 0.032,respectively,and polymorphic information content (PIC) was 0.0600.At the g.2998A ＞ G site located in exon 5,genotype frequencies of three genotypes AA,AG and GG were 0.632,0.300 and 0.068,respectively,and PIC was 0.2828.Both two sites were in Hardy-Weinberg equilibrium (P ＞ 0.05).The association analysis showed that individuals with genotype CC had higher average values than those individuals with genotype AA in the growth traits (body weight,body length,overall length and body height) at the g.2169A ＞ C site,but the difference was not significant (P ＞ 0.05).At the g.2998A ＞ G site,the average value of each growth trait was highest in the individuals of genotype AG,and lowest in the individuals with genotypes GG.However,only overall length between the individuals with genotype AG and GG had significant difference (P ＜ 0.05).These results imply that the mutations in the coding region of FST gene may have a certain effect on the growth traits in Larimichthys crocea.This result provides the basis for the choice of body shape in the breeding of Larimichthys crocea.关键词
大黄鱼/卵泡抑素/高分辨率熔解曲线/单核苷酸多态性/生长性状Key words
large yellow croaker/follistatin/high resolution melting curve/single nucleotide polymorphism/growth traits引用本文复制引用
张玉,韩明星,曹明月,赵亚运,李娟,薛良义..大黄鱼FST基因SNP筛选及与生长性状相关性分析[J].核农学报,2018,32(5):883-891,9.基金项目
国家自然科学基金(31172398),浙江省育种专项(2016C02055-7),宁波市重大专项(2015C110005) （31172398）
