核农学报2018，Vol.32Issue(6)：1050-1059,10.DOI:10.11869/j.issn.100-8551.2018.06.1050
中国南瓜果皮颜色基因连锁的SNP分子标记的开发及应用
Development and Application of SNP Molecular Marker Linked to Pericarp Color in Cucurbita moschata Duch.
摘要
Abstract
In order to understand the inheritance of pericarp color and develop SNP molecular markers that colosely linked to the pericarp color trait of pumpkin (Cucurbita moschata Duch.),genetic analysis and gene mapping were carried out on green pericarp color fruit using CMO-1 (dark green without stripe) and CMO-97 (light green with stripe) as experimental materials in this study.Meanwhile,dCAPS molecular markers that generated by SNP molecular markers have been developed and applied in the mapped interval.Results indicated that light green with stripe was dominate to dark green with no stripe,the pericarp color was controlled by single genes,designated as pc.Based on high-density genetic map constructed previously,a locus related to pericarp color and pericarp stripe traits of pumpkin was detected in linkage group 8,and both traits might be controlled by the same gene.The pc gene was mapped near R1 _47757 which had the highest phenotypic variance explained (PVE).The flanking markers R2_47028 and R2_63809 were linked to the pc gene with a genetic distance of 1.86 cM,and a physical distance of 145.13 kb.Bioinformatics analysis revealed that there were 32 prediction candidate genes in this region.At the same time,dCAPS primers were designed based on the information of SNP molecular markers related to pericarp color trait.After PCR amplification and restriction enzyme digestion,two dCAPS molecular markers with high amplification efficiency and stability were selected to distinguish dark green without stripe phenotype from light green with stripe in F2 population according to the digestion patterns,and used to identify the pericarp color of pumpkin inbred lines.These results laid the foundation for the molecular mechanism of pericarp color development and molecular marker-assisted breeding of Cucurbita moschata Duch.关键词
南瓜/果皮颜色/基因定位/SNP分子标记/分子标记辅助育种Key words
pumpkin/pericarp color/gene mapping/SNP molecular marker/molecular marker-assisted breeding引用本文复制引用
周洋洋,黄河勋,李俊星,罗少波,吴廷全,钟玉娟..中国南瓜果皮颜色基因连锁的SNP分子标记的开发及应用[J].核农学报,2018,32(6):1050-1059,10.基金项目
广东省省级科技计划项目(2015B070701014),国家自然科学基金青年基金(31601748),广州市科技计划项目(201707010386、201704020075),广州市农业产业发展专项基金(1710023) （2015B070701014）
